Effect of DHEA Administration Alone or Exercise combined with DHEA before Steroid Treatment on Rat Hindlimb Muscles.
10.4040/jkan.2009.39.3.321
- Author:
Myoung Ae CHOE
1
;
Gyeong Ju AN
Author Information
1. College of Nursing, Seoul National University, Seoul, Korea. machoe@snu.ac.kr
- Publication Type:Original Article ; English Abstract ; Research Support, Non-U.S. Gov't
- Keywords:
Steroids;
Dehydroepiandrosterone;
Exercise;
Hindlimb;
Muscular atrophy
- MeSH:
Animals;
Body Weight;
Dehydroepiandrosterone/*administration & dosage;
Hindlimb;
Male;
Muscle Contraction/drug effects;
Muscle, Skeletal/*drug effects/pathology;
Muscular Atrophy/chemically induced/*prevention & control;
*Physical Conditioning, Animal;
Rats;
Rats, Sprague-Dawley;
Steroids/*toxicity
- From:Journal of Korean Academy of Nursing
2009;39(3):321-328
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The purpose of this study was to determine the effect of Dehydroepiandrosterone (DHEA) administration alone or exercise combined with DHEA before steroid treatment on rat hindlimb muscles. METHODS: Male Sprague-Dawley rats were assigned to one of three groups: a steroid group (S, n=10) that had no treatment for 7 days before steroid treatment; a DHEA-steroid group (DS, n=8) that had 0.34 mmol/kg/day DHEA injection once a day for 7 days before steroid treatment and an exercise?steroid group (EDS, n=9) that ran on the treadmill combined with 0.34 mmol/kg/day DHEA injection for 7 days before steroid treatment. At 15 days all rats were anesthetized and soleus, plantaris and gastrocnemius muscles were dissected. Body weight, food intake, muscle weight, myofibillar protein content and cross-sectional area of the dissected muscles were determined. RESULTS: The DS group showed significant increases (p<.05) as compared to the steroid group in body weight, and muscle weight of gastrocnemius muscles. The EDS group showed significant increases (p<.05) as compared to the S group in body weight, muscle weight, myofibrillar protein content, and Type II fiber cross-sectional area of soleus, plantaris and gastrocnemius muscles. CONCLUSION: Exercise combined with DHEA administration before steroid treatment prevents steroid induced muscle atrophy, with exercise combined with DHEA administration being more effective than DHEA administration alone in preventing muscle atrophy.
