Sox9 Increases the Proliferation and Colony-forming Activity of Outer Root Sheath Cells Cultured In Vitro.
- Author:
Ge SHI
1
;
Kyung Cheol SOHN
;
Soo Yeon KIM
;
Eun Kyoung RYU
;
Yeon Suk PARK
;
Young LEE
;
Young Joon SEO
;
Jeung Hoon LEE
;
Chang Deok KIM
Author Information
- Publication Type:In Vitro ; Original Article
- Keywords: beta-catenin; Outer root sheath cells; Sox9
- MeSH: Adenoviridae; beta Catenin; Electrolytes; Hair; Hair Follicle; Homeostasis; Humans; Keratin-15; RNA, Messenger
- From:Annals of Dermatology 2011;23(2):138-143
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: beta-catenin plays a pivotal role in hair follicle development and hair growth cycle. OBJECTIVE: The aim of this study was to identify beta-catenin-regulated genes in cultured human hair outer root sheath (ORS) cells. METHODS: Primary cultured ORS cells were transduced with recombinant adenovirus expressing N-terminal truncated beta-catenin (constitutive active form), and beta-catenin-regulated genes were identified. RESULTS: Overexpression of the constitutively active form of beta-catenin led to induction of Sox9 expression at both mRNA and protein levels. To investigate the potential role of Sox9, we made the recombinant adenovirus expressing green fluorescent protein-tagged Sox9, and then transduced into cultured ORS cells. Interestingly, Sox9 induced the expression of keratin 15, increased the proliferation of ORS cells in vitro, and enhanced colony-forming activity. CONCLUSION: Our results suggest that Sox9 is a beta-catenin-regulated gene in ORS cells, and has potential importance in the regulation of hair follicle homeostasis.
