Polymerase Chain Reaction in the Diagnosis of Infectious Endophthalmitis.
- Author:
Sung Jin LEE
1
;
You Jeong CHOI
;
Yong Sung YOU
;
Hyoung Jun KOH
;
Joon H LEE
;
Sung Chul LEE
;
Jeon Han PARK
;
Oh Woong KWON
Author Information
1. Department of Ophthalmology, Soonchunhyang University, College of Medicine.
- Publication Type:Original Article
- Keywords:
Culture;
Diagnosis;
Endophthalmitis;
PCR(polymerase chain reaction)
- MeSH:
Aqueous Humor;
Bacteria;
Diagnosis*;
Endophthalmitis*;
Fungi;
Polymerase Chain Reaction*
- From:Journal of the Korean Ophthalmological Society
2001;42(4):600-606
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We evaluated the efficacy of the PCR which is known as more sensitive method than culture in the diagnosis of causal microorganisms of the infective endophthalmitis. METHODS: We used 0.3 ml of aqueous humor and 0.5 ml of vitreous sample in 3 cases of postoperative and 1 case of endogenous endophthalmitis for detecting causal microorganisms. To detect the bacteria we used universal, Gram positive and negative primers, and to detect the fungus we used fungal primer. RESULTS: Three cases of endophthalmitis, there was no bacteria in the bacterial culture for 10 days but PCR results identified causal microorganisms in all cases. CONCLUSIONS: PCR is effective in the fast and accurate diagnosis of infective endophthalmitis and especially in the culture-negative endophthalmitis.
