Comparison of Retinal Waveform between Normal and rd/rd Mouse.
- Author:
Jang Hee YE
1
;
Je Hoon SEO
;
Yong Sook GOO
Author Information
1. Department of Physiology, Chungbuk National University School of Medicine, Cheongju, Korea. ysgoo@chungbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Degenerate retina;
Retinal waveform;
Multielectrode array;
Bipolar cell;
Ganglion cell
- MeSH:
Animals;
Electric Stimulation;
Electrodes;
Ganglion Cysts;
Macular Degeneration;
Mice;
Microelectrodes;
Prostheses and Implants;
Retina;
Retinal Diseases;
Retinal Ganglion Cells;
Retinaldehyde;
Retinitis Pigmentosa;
Visual Prosthesis
- From:Korean Journal of Medical Physics
2008;19(3):157-163
- CountryRepublic of Korea
- Language:English
-
Abstract:
Retinal prosthesis is regarded as the most feasible method for the blind caused by retinal diseases such as retinitis pigmentosa or age-related macular degeneration. One of the prerequisites for the success of retinal prosthesis is the optimization of the electrical stimuli applied through the prosthesis. Since electrical characteristics of degenerate retina are expected to differ from those of normal retina, we investigated differences of the retinal waveforms in normal and degenerate retina to provide a guideline for the optimization of electrical stimulation for the upcoming prosthesis. After isolation of retina, retinal patch was attached with the ganglion cell side facing the surface of microelectrode arrays (MEA). 8x8 grid layout MEA (electrode diameter: 30micrometer, electrode spacing: 200micrometer, and impedance: 50 k omega at 1 kHz) was used to record in-vitro retinal ganglion cell activity. In normal mice (C57BL/6J strain) of postnatal day 28, only short duration (<2 ms) retinal spikes were recorded. In rd/rd mice (C3H/HeJ strain), besides normal spikes, waveform with longer duration (~100 ms), the slow wave component was recorded. We attempted to understand the mechanism of this slow wave component in degenerate retina using various synaptic blockers. We suggest that stronger glutamatergic input from bipolar cell to the ganglion cell in rd/rd mouse than normal mouse contributes the most to this slow wave component. Out of many degenerative changes, we favor elimination of the inhibitory horizontal input to bipolar cells as a main contributor for a relatively stronger input from bipolar cell to ganglion cell in rd/rd mouse.
