Effects of Human Adipose-Tissue Derived Stem Cell Infusion on the Immunological Consequences in Skin Allograft Mice.
10.4285/jkstn.2013.27.4.174
- Author:
Sang Chul LEE
1
;
Haejoung SUL
;
Sang Mook LEE
;
Say June KIM
Author Information
1. Department of Surgery, Daejeon St. Mary's Hospital, The Catholic University of Korea College of Medicine, Daejeon, Korea. sejoonkim@hanmail.net
- Publication Type:In Vitro ; Original Article
- Keywords:
Adipose tissue-derived stem cells;
Immunological tolerance;
Mesenchymal stromal cells;
Skin transplantation;
Immunosuppression
- MeSH:
Adipose Tissue;
Animals;
Graft Survival;
Humans*;
Immunosuppression;
Interleukin-10;
Interleukins;
Lymphocyte Culture Test, Mixed;
Male;
Mesenchymal Stromal Cells;
Mice*;
Models, Animal;
Organ Transplantation;
RNA, Messenger;
Skin Transplantation;
Skin*;
Stem Cells*;
Tissue Donors;
Transplantation, Homologous*;
Transplants;
Tumor Necrosis Factor-alpha
- From:The Journal of the Korean Society for Transplantation
2013;27(4):174-184
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Many in vitro experiments have demonstrated the immunosuppressive properties of mesenchymal stem cells (MSCs). However, such properties have not yet been fully established in an in vivo setting. The purpose of this study was to determine immunosuppressive and anti-inflammatory properties of MSCs in a preclinical animal model in order to pave the way for replacement of conventional immunosuppressive therapy. METHODS: Male C57BL/6 mice and male BALB/c mice were chosen as skin graft donors and recipients, respectively. After performance of full-thickness skin transplantation on the back of mice, adipose tissue derived stem cells (1.0x10(6)/0.1 mL) stained with 4, 6-diamidino-2-phenylindole were transplanted into adipose tissue derived stem cell (ASC)-infused mice and phosphate buffered saline (PBS; 0.1 mL) was infused into PBS-infused mice. Immunological properties and graft survival were accessed and compared. RESULTS: The serum levels of proinflammatory interleukin (IL)-6 showed a decrease in ASC-infused mice compared to PBS-infused mice (P<0.005). In addition, interferon-lambda, IL-10, and tumor necrosis factor-alpha mRNA levels in the skin graft showed a decrease in ASC-infused mice, although without statistical significance. In ASC-infused mice, donor specific hyporesponsiveness was identified in a mixed lymphocyte reaction assay at 30 days after transplantation. In addition, ASC-infusion resulted in markedly prolonged skin allograft survival compared with PBS-infusion (P<0.001). CONCLUSIONS: Administration of ASC not only induced anti-inflammation and immunosuppression, but also resulted in prolonged graft survival, suggestive of their potent immunosuppressive properties. Therefore, conduct of further and more exquisite studies will be required in order to determine the role of MSC in the solid organ transplantation field in order to avoid adverse effects and toxicities caused by chemical immunosuppressive regimens.
