Inhibitory Effects of Imatinib Mesylate on Transdifferentiation of Lens Epithelial Cells into Fibroblast.
- Author:
Tea Sung PARK
1
;
Jun Seok TEA
;
Seh Min PARK
;
Jung Hyub OH
Author Information
1. Department of Ophthalmology, Inha University, College of Medicine, Inchon, Korea. jhoh9707@hanmail.net
- Publication Type:Original Article
- Keywords:
alpha-Smooth muscle actin;
Capsular bag model;
Fibroblast;
Imatinib mesylate;
Transdifferentiation
- MeSH:
Acceleration;
Bromodeoxyuridine;
Epithelial Cells*;
Fibroblasts*;
Mesylates*;
Transforming Growth Factor beta;
Imatinib Mesylate
- From:Journal of the Korean Ophthalmological Society
2005;46(2):257-270
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate whether Imatinib mesylate (IM) could inhibit the transdifferentiation of the lens epithelial cells (LECs) into fibroblasts using the capsular bag model. METHODS: In the capsular bag model, LECs were cultured by exposure to IM at various concentrations for 3 min. The effect of IM was analysed by observing the covering area, numbers of alpha-SMA positive cell, and BrdU incorporated proliferating cells. The same analysis was performed in the culture of LECs with TGF-beta. RESULTS: The covering area was significantly decreased by the treatment of 30 micro M IM, and the positive cells for alpha-SMA and BrdU were also decreased by IM treatment in a dose dependent manner. In addition, increasing TGF-beta concentration accelerated transdifferentiation, but suppressed the acceleration of the transdifferentiation induced by TGF-beta. CONCLUSIONS: In the capsular bag model, IM effectively inhibited not only the transdifferentiation of LECs into fibroblasts but also the TGF-beta induced acceleration of the transdifferentiation.
