Effect of Cryotherapy on Focal Adhesion Kinase in Retinal Pigment Epithelial Cells.
- Author:
Jun Gyo LEE
1
;
Jang Won HEO
;
Hum CHUNG
Author Information
1. Seoul Eye Clinic, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Cryotherapy;
Focal adhesion kinase;
Proliferative vitreoretinopathy;
Retinal tear
- MeSH:
Apoptosis;
Basement Membrane;
Cryotherapy*;
Epithelial Cells*;
Focal Adhesion Protein-Tyrosine Kinases*;
Focal Adhesions*;
Phosphotransferases;
Rabbits;
Retinal Perforations;
Retinaldehyde*;
Vitrectomy;
Vitreoretinopathy, Proliferative
- From:Journal of the Korean Ophthalmological Society
2005;46(2):360-365
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: In the pathogenesis of proliferative vitreoretinopathy (PVR), retinal pigment epithelial (RPE) cells do not undergo apoptosis after separation from the basement membrane. In order to investigate the mechanism by which RPE cells resist apoptosis in PVR, the expression of focal adhesion kinase after cryotherapy was studied. METHODS: After lensectomy and vitrectomy was performed on rabbit eyes, a retinal tear was created. Cryotherapy was applied to the tear site one week after the tear creation. Rabbits were sacrificed at 1, 4 and 7 days after the tear formation and 30 minutes, 1, 4, 7, 9, 21 days after cryotherapy. Eyes were stained with anti-focal adhesion kinase (FAK) antibody. RESULTS: Expression of FAK increased after the tear formation and remained increased until 21days after cryotherapy. CONCLUSIONS: These data show that creation of a retinal tear and cryotherapy increases FAK expression and may contribute to resistance to apoptosis.
