Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regeneration.
10.1186/s40902-016-0062-4
- Author:
Chae Kyung YOO
1
;
Jae Yun JEON
;
You Jin KIM
;
Seong Gon KIM
;
Kyung Gyun HWANG
Author Information
1. Department of Translational Medicine, Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, South Korea. hkg@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
Bone regeneration;
Cell adhesion;
Cell proliferation;
Membrane;
Osteoblasts;
Silk fibroin
- MeSH:
Bone Regeneration*;
Cell Adhesion;
Cell Proliferation;
Cell Survival;
Fibroins*;
Fluorescence;
Membranes*;
Microscopy;
Microscopy, Electron, Scanning;
Osteoblasts;
Silk*
- From:Maxillofacial Plastic and Reconstructive Surgery
2016;38(3):17-
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: The aim of this study is to verify the feasibility of using silk fibroin (SF) as a potential membrane for guided bone regeneration (GBR). METHODS: Various cellular responses (i.e., cell attachment, viability, and proliferation) of osteoblast-like MG63 cells cultured on an SF membrane were quantified. After culturing on an SF membrane for 1, 5, and 7 days, the attachment and surface morphology of MG63 cells were examined by optical and scanning electron microscopy (SEM), cell viability was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell proliferation was quantified using 4',6-diamidino-2-phenylindole (DAPI) fluorescence staining. RESULTS: Optical microscopy revealed that MG63 cells cultured on the SF membrane proliferated over the 7-day observation period. The viability of cells cultured on SF membranes (SF group) and on control surfaces (control group) increased over time (P < 0.05); however, at respective time points, cell viability was not significantly different between the two groups (P > 0.05). In contrast, cell proliferation was significantly higher in the SF membrane group than in the control group at 7 days (P < 0.05). CONCLUSIONS: These results suggest that silk fibroin is a biocompatible material that could be used as a suitable alternative barrier membrane for GBR.
