Therapeutic effect of ethyl acetate extract from Asparagus cochinchinensis on phthalic anhydride-induced skin inflammation.
- Author:
Ji Eun SUNG
1
;
Hyun Ah LEE
;
Ji Eun KIM
;
Jun GO
;
Eun Ji SEO
;
Woo Bin YUN
;
Dong Seob KIM
;
Hong Joo SON
;
Chung Yeoul LEE
;
Hee Seob LEE
;
Dae Youn HWANG
Author Information
- Publication Type:Original Article
- Keywords: Asparagus cochinchinensis; skin inflammation; IL-4/Luc/CNS-1 transgenic mice; phthalic anhydride; IgE
- MeSH: Animals; Biomarkers; Brain Diseases; Cough; Epidermis; Fever; Homeostasis; Immunoglobulin E; Immunoglobulins; Inflammation*; Inflammatory Breast Neoplasms; Interleukin-4; Kidney Diseases; Luciferases; Lymph Nodes; Mast Cells; Mice; Phenotype; Skin*
- From:Laboratory Animal Research 2016;32(1):34-45
- CountryRepublic of Korea
- Language:English
- Abstract: Asparagus cochinchinensis has been used to treat various diseases including fever, cough, kidney disease, breast cancer, inflammatory disease and brain disease, while IL-4 cytokine has been considered as key regulator on the skin homeostasis and the predisposition toward allergic skin inflammation. However, few studies have investigated its effects and IL-4 correlation on skin inflammation to date. To quantitatively evaluate the suppressive effects of ethyl acetate extracts of A. cochinchinensis (EaEAC) on phthalic anhydride (PA)-induced skin inflammation and investigate the role of IL-4 during their action mechanism, alterations in general phenotype biomarkers and luciferase-derived signals were measured in IL-4/Luc/CNS-1 transgenic (Tg) mice with PA-induced skin inflammation after treatment with EaEAC for 2 weeks. Key phenotype markers including lymph node weight, immunoglobulin E (IgE) concentration, epidermis thickness and number of infiltrated mast cells were significantly decreased in the PA+EaEAC treated group compared with the PA+Vehicle treated group. In addition, expression of IL-1β and TNF-α was also decreased in the PA+EaEAC cotreated group, compared to PA+Vehicle treated group. Furthermore, a significant decrease in the luciferase signal derived from IL-4 promoter was detected in the abdominal region, submandibular lymph node and mesenteric lymph node of the PA+EaEAC treated group, compared to PA+Vehicle treated group. Taken together, these results suggest that EaEAC treatment could successfully improve PA-induced skin inflammation of IL-4/Luc/CNS-1 Tg mice, and that IL-4 cytokine plays a key role in the therapeutic process of EaEAC.
