The Regulation of Cyclooxygenase-2 Expressionby Interleukin-1beta in WISH cells.
- Author:
Young Jin CHANG
1
;
Yoon Ki PARK
;
Suk Whan BAEK
;
Young Ki LEE
;
Dong Hyuk LEE
;
Hyun Woo LEE
Author Information
1. Department of Obstetrics and Gynecology, Yeungnam University College of Medicine, Taegu, Korea.
- Publication Type:Original Article
- Keywords:
WISH cell;
Interleukin-1beta (IL-1beta);
cyclooxygenase-2 (COX-2);
protein kinase C (PKC);
phosphatidylinositol 3-kinase (PI 3K)
- MeSH:
Amnion;
Blotting, Western;
Culture Media;
Cycloheximide;
Cyclooxygenase 2*;
Dactinomycin;
Dinoprostone;
Immunoenzyme Techniques;
Interleukin-1beta*;
Phosphatidylinositol 3-Kinase;
Protein Kinase C
- From:Korean Journal of Obstetrics and Gynecology
2001;44(8):1393-1400
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To determine of the regulation of cyclooxygenase-2 (COX-2) expression by Interleukin-1beta in WISH cells. METHODS: Amnion WISH cells were incubated in media containing increasing concentrations of IL-1beta or with various inhibitors. Increased COX-2 expression was determined by Western blot analysis with anti-COX-2 antibody. Concomitant measurements of culture media PGE2 were made by an enzyme immunoassay. RESULTS: The COX-2 and prostaglandin E2 production induced by IL-1beta increased in a dose- and time-dependent manner. One of the regulating factors that induced COX-2 by IL-1beta was protein kinase C (PKC). PKC inhibitor, Ro 31-8220 was pretreated and continued treating by IL-1beta. Then, PKC inhibitor completely blocked COX-2 protein induction by IL-1beta. In contrast, COX-2 induction by IL-1beta after pretreating PKC stimulator, phobol 12-myristate 13-acetate was potentiated with synergism. Another factor in controlling COX-2 protein induction was identified as phosphatidylinositol 3-kinase (PI 3K). COX-2 protein induction by IL-1beta after pretreating PI 3K inhibitors, wortmannin and LY294002 strongly increased. This kind of result reflected that PI 3K act as negative regulator. COX-2 induction by IL-1beta was known to be regulated in not only transcription step, but also translation step after performing experiment of actinomycin and cycloheximide treatment. CONCLUSION: COX-2 protein and prostaglandin E2 production induced by IL-1beta were controlled by many factors in amnion cell. Among those factors, PKC and PI 3K have an important role, but their control mechanism act as positive and negative, respectively.
