Establishment of Mouse Oocytes Cryopreservation Program.
- Author:
Shin Yong MOON
1
;
Seok Hyun KIM
;
Byung Chul JEE
;
Ki Cheong SUNG
;
Sung Mi CHOI
;
Hee Sun KIM
;
Sun Kyung OH
;
Chang Suk SUH
;
Young Min CHOI
;
Jung Gu KIM
;
Jin Yong LEE
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Mouse oocytes;
Cryopreservation;
Slow freezing and ultra-rapid thawing;
Vitrification
- MeSH:
Animals;
Cryopreservation*;
Dimethyl Sulfoxide;
Ethylene Glycol;
Fertilization;
Freezing;
Mice*;
Oocytes*;
Propylene Glycol;
Sucrose;
Survival Rate;
Vitrification
- From:Korean Journal of Obstetrics and Gynecology
2001;44(8):1455-1463
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To establish the optimal cryopreservation method in mouse oocytes. METHODS: Firstly, mouse immature oocytes were exposed to various cryoprotectants, and then cryoprotectant with the best outcome was selected. Secondly, mouse immature oocytes were cryopreserved by either slow freezing and ultra-rapid thawing or vitrification. Finally, in mouse mature oocytes, the five different protocols were compared in their fertilization and hatching rates. RESULTS: 1) 1.5M 1,2-propanediol (PROH) and 1.5M PROH+0.1M sucrose had a higher rate of survival (73.1%, 81.9%) and in vitro maturation (28.2%, 30.1%). 2) Vitrification using 5.5M ethylene glycol (EG) showed significantly higher rate of survival and in vitro maturation, when compared with slow freezing and ultra-rapid thawing using 1.5M PROH+0.1M sucrose (65.9% vs 50.0%, 40.0% vs 28.2%, respectively). 3) In mouse mature oocytes, vitrification using 5.5M EG showed significantly higher survival rate, however, slow freezing and ultra-rapid thawing using 1.5M DMSO was superior to vitrification in view of fertilization rate. CONCLUSIONS: Vitrification showed better outcomes in mouse immature oocytes, but slow freezing and ultra-rapid thawing using 1.5M DMSO may be beneficial in mature oocytes.
