Canine adipose tissue-derived mesenchymal stem cells ameliorate severe acute pancreatitis by regulating T cells in rats.
10.4142/jvs.2016.17.4.539
- Author:
Hyun Wook KIM
1
;
Woo Jin SONG
;
Qiang LI
;
Sei Myoung HAN
;
Kee Ok JEON
;
Sang Chul PARK
;
Min Ok RYU
;
Hyung Kyu CHAE
;
Kweon KYEONG
;
Hwa Young YOUN
Author Information
1. Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea. hyyoun@snu.ac.kr
- Publication Type:Original Article
- Keywords:
acute pancreatitis;
anti-inflammatory agents;
dogs;
mesenchymal stromal cell;
regulatory T-lymphocytes
- MeSH:
Acinar Cells;
Animals;
Anti-Inflammatory Agents;
Cytokines;
Dogs;
Edema;
Inflammation;
Interleukin-10;
Interleukin-4;
Interleukins;
Mesenchymal Stromal Cells*;
Models, Animal;
Mortality;
Necrosis;
Pancreas;
Pancreatic Ducts;
Pancreatitis*;
Rats*;
Real-Time Polymerase Chain Reaction;
T-Lymphocytes*;
T-Lymphocytes, Regulatory;
Taurocholic Acid
- From:Journal of Veterinary Science
2016;17(4):539-548
- CountryRepublic of Korea
- Language:English
-
Abstract:
Severe acute pancreatitis (SAP) is associated with systemic complications and high mortality rate in dogs. Mesenchymal stem cells (MSCs) have been investigated for their therapeutic potential in several inflammation models. In the present study, the effects of canine adipose tissue-derived (cAT)-MSCs in a rat model of SAP induced by retrograde injection of 3% sodium taurocholate solution into the pancreatic duct were investigated. cAT-MSCs labeled with dioctadecyl-3,3,3′-tetramethylindo-carbocyanine perchlorate (1 × 10⁷ cells/kg) were systemically administered to rats and pancreatic tissue was collected three days later for histopathological, quantitative real-time polymerase chain reaction, and immunocytochemical analyses. Greater numbers of infused cAT-MSCs were detected in the pancreas of SAP relative to sham-operated rats. cAT-MSC infusion reduced pancreatic edema, inflammatory cell infiltration, and acinar cell necrosis, and decreased pancreatic expression of the pro-inflammatory cytokines tumor necrosis factor-α, interleukin (IL)-1β, -6, -12, -17, and -23 and interferon-γ, while stimulating expression of the anti-inflammatory cytokines IL-4 and IL-10 in SAP rats. Moreover, cAT-MSCs decreased the number of clusters of differentiation 3-positive T cells and increased that of forkhead box P3-positive T cells in the injured pancreas. These results indicate that cAT-MSCs can be effective as a cell-based therapeutic strategy for treatment of SAP in dogs.
