The Relationship between Expression of 1-Cys Peroxiredoxin and Resistance to Cisplatin in Epithelial Ovarian Cancer Cell Lines.
- Author:
Won Duk JOO
1
;
Jang Ho PAK
;
Jong Hyeok KIM
;
Shin Hyung LEE
;
Dae Yeon KIM
;
Dae Shik SUH
;
Yong Man KIM
;
Young Tak KIM
;
Jung Eun MOK
;
Joo Hyun NAM
Author Information
1. Department of Obstetrics and Gynecology, University of Ulsan, College of Medicine, Seoul, Korea. hyeokkim@amc.seoul.kr
- Publication Type:Original Article
- Keywords:
Epithelial ovarian cancer;
1-Cys peroxiredoxin;
Cisplatin;
Reactive oxygen species;
Resistance
- MeSH:
Antioxidants;
Apoptosis;
Blotting, Western;
Cell Line*;
Cisplatin*;
Cytoplasm;
Epithelial Cells;
Epithelium;
Female;
Immunohistochemistry;
In Situ Nick-End Labeling;
Ovarian Neoplasms*;
Ovary;
Peroxiredoxins*;
Plasmids;
Reactive Oxygen Species
- From:Korean Journal of Obstetrics and Gynecology
2006;49(10):2137-2147
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate the relationship between expression of 1-Cys peroxiredoxin (Prx) and resistance to cisplatin in epithelial ovarian cancer cell lines. METHODS: Immunohistochemistry of 1-Cys Prx was performed on both normal ovarian tissues and the tissues of epithelial ovarian cancer. Western blot was performed to measure the expression of 1-Cys Prx in SKOV-3, OVCAR-3 and SNU-8 after treatment with cisplatin. Expression of 1-Cys Prx in SKOV-3 was also measured according to both time after treatment with cisplatin and concentration of cisplatin. The generation of reactive oxygen species (ROS) was measured with and without antioxidants in SKOV-3. SKOV-3 was transfected with 1-Cys Prx green fluorescent protein plasmid to overexpress 1-Cys Prx and TUNEL assay was performed after treatment with cisplatin to examine apoptosis. RESULTS: 1-Cys Prx was strongly expressed in both stroma and epithelium of both normal ovary and epithelial ovarian cancer, especially in the cytoplasm of epithelial cells. SNU-8 and OVCAR-3 exhibited about 1.5 fold higher expression than SKOV-3. SKOV-3 showed the peak expression at 48 hours after treatment with cisplatin and in 3 microgram/mL concentration of cisplatin. The generation of ROS was increased after treatment with cisplatin to SKOV-3 and the survival of SKOV-3 against cisplatin was correlated with the concentration of antioxidants (p<0.001). No apoptosis occurred in 1-Cys Prx overexpressed SKOV-3 cells. CONCLUSION: 1-Cys Prx was shown to increase the resistance to cisplatin in epithelial ovarian cancer cell line. The result suggests that the resistance may be due to overexpression of 1-Cys Prx, which is responsible for removal of ROS generated by cisplatin.
