The changes of fibronectin in the cultured porcine lens epithelial cells.
- Author:
Sung Bum HONG
1
;
Jae Hwan LEE
;
Myung Jin KIM
;
Jung Hyub OH
Author Information
1. Department of Ophthalmology, Medical College, Inha University, Korea.
- Publication Type:Original Article
- Keywords:
bFGF (basic fibroblast growth factor);
Cataract;
Fibronectin;
Lens epithelial cells
- MeSH:
Blotting, Western;
Cataract;
Cornea;
Cytoplasm;
Epithelial Cells*;
Epithelium, Corneal;
Extracellular Matrix;
Fibronectins*;
Immunohistochemistry;
Lens, Crystalline
- From:Journal of the Korean Ophthalmological Society
2003;44(1):195-201
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: to investigate change of fibronectin as a relating factor of cellular migration in the cultured lens epithelial cells. METHODS: We attempted to observe the fibronectin from cornea, crystalline lens and cultured lens epithelial cells by immunocytochemistry. We also compared the expression of fibronectin from normal porcine crystalline lens epithelial cells and cultured lens epithelial cells that received bFGF with western blot method. RESULTS: Fibronectin was detected in the extracellular matrix of normal corneal epithelium by immunocytochemistry, but not in the normal lens epithelial cells. However, fibronectin was detected in the cytoplasm of the cultured lens epithelial cells and treated with bFGF by immunocytochemistry. CONCLUSIONS: lens epithelial cells in normal condition may not produce fibronectin, but lens epithelial cells without lens nucleus after cataract surgery produce fibronectin.
