Expression of Cdc25A, Cdc25B and Cdc25C in cervical carcinoma.
- Author:
Hye Yeon KIM
1
;
Jae Wook KIM
;
Sei Kwang KIM
;
Young Tae KIM
;
Jae Hoon KIM
;
Sung Hoon KIM
;
Sang Wun KIM
;
Bo Sung YOON
;
Eun Ji NAM
;
Hye Jin CHO
Author Information
1. Department of Obstetrics and Gynecology, Yonsei University College of Medicine, Seoul, Korea. ytkchoi@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Cervical carcinoma;
Cdc25A;
Cdc25B;
Cdc25C;
RT-PCR;
Western blot analysis
- MeSH:
Blotting, Western;
Carcinogenesis;
cdc25 Phosphatases;
Cell Cycle;
Cervix Uteri;
DNA;
Female;
Flow Cytometry;
Genital Diseases, Female;
Humans;
Lymph Nodes;
Neoplasm Metastasis;
RNA, Messenger;
Survival Rate;
Uterine Cervical Neoplasms
- From:Korean Journal of Obstetrics and Gynecology
2006;49(10):2156-2165
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: This study was undertaken to quantitatively detect Cdc25A, Cdc25B and Cdc25C in cervical carcinoma and determine the relationship between the expression of mRNA and protein of cell division cycle (Cdc)25 phosphatase and various clinicopathologic prognostic factors of cervical carcinoma. METHODS: 39 patients diagnosed with cervical carcinoma between February 2000 to March 2005 and 10 patients with benign gynecologic disease were enrolled in this study. A reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis were used to analyze the expression of Cdc25 phosphatase mRNA and protein in fresh invasive cervical cancer tissue and normal cervix tissue. RESULTS: The mRNA expressions of Cdc25A, Cdc25B and Cdc25C in the cancer tissues were significantly greater than in the control (p=0.02, 0.01, 0.02), respectively. A Western blot analysis yielded same results (p=0.01, 0.02, 0.01). There were also significant relationships between the age and the Cdc25B mRNA expression (p=0.03), between the cell type and the Cdc25C mRNA expression (p=0.04). However, other clinicopathologic prognostic factors including stage, subtype, SCC Ag level, DNA flow cytometry, lymph node metastasis, lymphovascular space invasion and HPV positivity were not statistically significant. CONCLUSION: Our results show that Cdc25A, Cdc25B and Cdc25C expression levels were significantly greater in cervical cancer patient group than in those of control group. Thus Cdc25 phosphatase might play an important role in carcinogenesis of cervical carcinoma. Further studies based on the correlation between Cdc25 phosphatase and survival rate would be need to support Cdc25 phosphatase as a prognostic factor of cervical carcinoma.
