Pro-apoptotic Cytochrome c Gene Mutation is Rare in Non-small Cell Lung Cancers.
- Author:
Young Hwa SOUNG
1
;
Sug Hyung LEE
Author Information
- Publication Type:Original Article
- Keywords: Non-small cell lung cancer; Cytochrome c; Apoptosis; Mutation
- MeSH: Apoptosis; Base Sequence; Carcinoma, Non-Small-Cell Lung; Clinical Coding; Cytochromes c*; Cytochromes*; Humans; Lung Neoplasms*; Lung*; Oxidative Phosphorylation; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Sequence Analysis, DNA
- From:Journal of Lung Cancer 2006;5(2):111-113
- CountryRepublic of Korea
- Language:Korean
- Abstract: PURPOSE: Several lines of evidence have indicated that the deregulation of apoptosis is involved in the mechanisms of cancer development, and somatic mutations of the apoptosis-related genes have been reported in human cancers. In addition to its role in oxidative phosphorylation, release of cytochrome c from the mitochondrial intermembrane space results in nuclear apoptosis. The aim of this study was to explore whether alteration of cytochrome c gene mutation is a characteristic of human non-small cell lung cancers (NSCLC). MATERIALS AND METHODS: In the current study, to detect the somatic mutations in the DNA sequences encoding cytochrome c in 48 NSCLCs, we used polymerase chain reaction (PCR), single-strand conformation polymorphism (SSCP), and DNA sequencing. RESULTS: The SSCP analysis revealed no mutation in the entire coding regions and all splice sites of human cytochrome c gene in the 48 NSCLCs. CONCLUSION: The data presented here indicate that the pro-apoptotic cytochrome c may not be somatically mutated in human NSCLCs, and suggest that NSCLCs may not utilize mutational events of cytochrome c gene in the mechanisms for evading apoptosis.
