Apoptosis and Expression of bcl-2, p53, and Ki-67 in Pilomatricoma.
- Author:
Chea Wook LEE
1
;
Ho Sun JANG
;
Chang Keun OH
;
Kyung Sool KWON
Author Information
1. Department of Dermatology, College of Medicine, Pusan National University, Pusan, Korea.
- Publication Type:Original Article
- Keywords:
Pilomatricoma;
Apoptosis;
Bcl-2;
p53;
Ki-67
- MeSH:
Antibodies, Monoclonal;
Apoptosis*;
Basophils;
Biopsy;
Cell Differentiation;
Hair;
In Situ Nick-End Labeling;
Pilomatrixoma*;
Skin
- From:Korean Journal of Dermatology
1999;37(11):1560-1566
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Pilomatricoma is a benign epidermal appendage tumor with differentiation toward hair matrix cells. Histologically, pilomatricoma comprises masses of immature basophilic cells, few transitional cells, and clusters of shadow cells. The mechanism leading to the formation of shadow cells is still unknown. OBJECTIVE: The aim of this study is to examine the expression of p53, bcl-2, Ki-67, and apoptotic rate for the investigation of the cell turnover and cell differentiation within pilomatricoma. METHODS: Immunohistochemical staining(LSAB technique) using monoclonal antibodies including bcl-2, p53, and Ki-67(MIB-1) is performed on skin biopsy specimens of pilomatricoma, and TUNEL staining for detecting apoptotic cells is also performed. RESULTS: The expression of Ki-67 and bcl-2 is noted in basal basophilic cells more than overlying basophilic cells. The p53 protein is observed to be alike on basal and overlying basophilic cells. But apoptotic cells are only expressed in transitional cells. CONCLUSION: The result of this study suggests that high proliferative area, such as basal basophilic cells manifested by over-expression of Ki-67 and bcl-2, is regulated by the p53 protein inducing apoptosis. Thereafter, basophilic cells may progress to shadow cells through apoptotic transitional cells by the action of p53 protein.
