The Role of Nitric Oxide (NO) in UVB-induced Apoptosis in HaCaT Keratinocytes.
- Author:
Kyung Jeh SUNG
1
;
Eun Mi PAIK
;
Mi Jung KIM
;
Chi Woo SUH
;
Ho Seok SUH
;
Jee Ho CHOI
Author Information
1. Department of Dermatology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
HaCaT cells;
Apoptosis;
UVB;
Nitric oxide
- MeSH:
Apoptosis*;
Keratinocytes*;
Nitric Oxide Synthase;
Nitric Oxide*;
omega-N-Methylarginine;
Propidium
- From:Korean Journal of Dermatology
1999;37(11):1596-1602
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: According to target cells, apoptosis-inducing agents, and NO concentration, NO concentration, NO shows both pro- and antiapoptotic effects. OBJECTS: Our study was perfermed to verify the role of NO in UVB-induced apoptosis in HaCaT cells. METHODS: After UVB irradiation, FACS using propidium iodide, LDH cytotoxicity assay, and nitrite assay based on Griess reaction were done in HaCaT cells. These procedures were repeated after UVB irradiation and NG-monomethyl-arginine (L-NMMA), a NO synthase (NOS) inhibitor, addition. RESULTS: 1) UVB irradiation (5-80mJ/cm2) induced apoptosis in HaCaT cells dose-dependently. 2) UVB irradiation (80mJ/cm2) stimulated NO production 30-50% more over baseline level, and this was inhibited by 500 micrometer L-NMMA. 3) 500 micrometer L-NMMA did not inhibit UVB-induced apoptosis. CONCLUSION: UVB irradiation evokes apoptosis in HaCaT keratinocytes through NO-independent mechanism.
