Cytotoxic Activity from Curcuma zedoaria Through Mitochondrial Activation on Ovarian Cancer Cells.
- Author:
Yujin SHIN
1
;
Yongkyu LEE
Author Information
1. Department of Food and Nutrition, Dongseo University, Busan, Korea. lyk@dongseo.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Cytotoxic activity;
alpha-Curcumene;
Mitochondrial activation;
ovarian cancer cells
- MeSH:
Apoptosis;
Caspase 3;
Caspases;
Cell Death;
Cell Survival;
Curcuma*;
Cytochromes c;
DNA Fragmentation;
Humans;
Ovarian Neoplasms*
- From:Toxicological Research
2013;29(4):257-261
- CountryRepublic of Korea
- Language:English
-
Abstract:
alpha-Curcumene is one of the physiologically active components of Curcuma zedoaria, which is believed to perform anti-tumor activities, the mechanisms of which are poorly understood. In the present study, we investigated the mechanism of the apoptotic effect of alpha-curcumene on the growth of human overian cancer, SiHa cells. Upon treatment with alpha-curcumene, cell viability of SiHa cells was inhibited > 73% for 48 h incubation. alpha-Curcumene treatment showed a characteristic nucleosomal DNA fragmentation pattern and the percentage of sub-diploid cells was increased in a concentration-dependent manner, hallmark features of apoptosis. Mitochondrial cytochrome c activation and an in vitro caspase-3 activity assay demonstrated that the activation of caspases accompanies the apoptotic effect of alpha-curcumene, which mediates cell death. These results suggest that the apoptotic effect of alpha-curcumene on SiHa cells may converge caspase-3 activation through the release of mitochondrial cytochrome c.
