Ginsenoside Rb1 attenuates intestinal ischemia-reperfusion- induced liver injury by inhibiting NF-kappa B activation.
10.3858/emm.2008.40.6.686
- Author:
Jin WANG
1
;
Lifen QIAO
;
Yongsheng LI
;
Guangtian YANG
Author Information
1. Department of Emergency Medicine, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, Hubei 430030, P.R. China. Guangtiany@hotmail.com
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
ginsenoside Rb1;
intercellular adhesion molecule-1;
NF-kappa B;
reperfusion injury;
tumor necrosis factor-alpha
- MeSH:
Alanine Transaminase/blood;
Animals;
Aspartate Aminotransferases/blood;
Biological Markers/metabolism;
Ginsenosides/*pharmacology;
Intercellular Adhesion Molecule-1/metabolism;
Intestines/*blood supply/metabolism/pathology;
Ischemia/metabolism/pathology;
Liver/enzymology/pathology;
Liver Diseases/etiology/*pathology;
Male;
Malondialdehyde/metabolism;
NF-kappa B/*antagonists & inhibitors/metabolism;
Peroxidase/metabolism;
Rats;
Rats, Wistar;
Reperfusion Injury/complications/*pathology;
Tumor Necrosis Factor-alpha/metabolism
- From:Experimental & Molecular Medicine
2008;40(6):686-698
- CountryRepublic of Korea
- Language:English
-
Abstract:
Intestinal ischemia-reperfusion (I/R) is an important event in the pathogenesis of multiple organ dysfunction syndrome (MODS). The aim of this study is to determine the effects of ginsenoside Rb1 on liver injury induced by intestinal I/R in rats. Adult male Wistar rats were randomly divided into four groups: (1) a control, sham-operated group (sham group); (2) an intestinal I/R group subjected to 1 h intestinal ischemia and 2 h reperfusion (I/R group); (3) a group treated with 20 mg/kg ginsenoside Rb1 before reperfusion (Rb1-20 group); and (4) a group treated with 40 mg/kg ginsenoside Rb1 before reperfusion (Rb1-40 group). Liver and intestinal histology was observed. Aspartate aminotransferase (AST), alanine aminotransferase (ALT) level in serum and malondialdehyde (MDA) level in intestinal tissues were measured. Myeloperoxidase (MPO), TNF-alpha, MDA level and immunohistochemical expression of NF-kappa B and intracellular adhesion molecale-1 (ICAM-1) in liver tissues was assayed. In addition, a western blot analysis of liver NF-kappa B expression was performed. Results indicated intestinal I/R induced intestinal and liver injury, which was characterized by increase of AST and ALT in serum, MDA level in intestine, MPO, TNF-alpha and MDA level and ICAM-1 and NF-kappa B expression in the liver tissues. Ginsenoside Rb1 (20, 40 mg/kg) ameliorated liver injury, decreased MPO, TNF-alpha and MDA level, NF-kappa B and ICAM-1 expression in liver tissues. In conclusion, ginsenoside Rb1 ablated liver injury induced by intestinal I/R by inhibiting NF-kappaB activation.
