Diagnostic Utility of Polymerase Chain Reaction-Based Clonality Analysis for Immunoglobulin Heavy Chain Gene and T-cell Receptor Gamma Chain Gene Rearrangement in Lymphoid Neoplasms.
- Author:
Eun Yoon CHO
1
;
Young Hyeh KO
;
Dae Shick KIM
;
Jae Joon HAN
;
Howe J REE
Author Information
1. Department of Diagnostic Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Korea. yhko@smc.samsung.co.kr
- Publication Type:Original Article
- Keywords:
Lymphoma;
Polymerase Chain Reaction
- MeSH:
Consensus;
Diagnosis;
Gene Rearrangement*;
Genes, T-Cell Receptor;
Heteroduplex Analysis;
Humans;
Immunoglobulin Heavy Chains*;
Immunoglobulins*;
Lymphoma;
Paraffin;
Polymerase Chain Reaction;
Receptors, Antigen, T-Cell*;
T-Lymphocytes*
- From:Korean Journal of Pathology
2001;35(6):461-469
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The clonality of lymphoid infiltrates determined by polymerase chain reaction (PCR) for immunoglobulin heavy chain (IgH) or T cell receptor (TCR) genes is not only useful in confirming the diagnosis of malignant lymphoma but also in establishing the lineage of a clonal lymphoid proliferation. We analyzed the efficiency of PCR analyses for IgH and TCRgenes that have been routinely applied for the diagnosis of malignant lymphoma in our laboratory. METHODS: Paraffin sections of 200 cases were analyzed by seminested PCR. Primers were FRIIIA-LJH/VLJH consensus primer for IgH gene and V-J consensus primer for TCR gene. The cases showing negative results by PCR for TCR gene were further analyzed by multiplex V family primers with heteroduplex analysis. RESULTS: PCR approach for IgH gene allowed detection of clonality in 100% of cases with false positive rate of 0.3% and false negative rate of 0%. The combination of PCR for TCR consensus primers with multiplex V family primers allowed detection of clonality in 91% of cases with false positive rate of 0.6% and false negative rate of 10.3%. CONCLUSIONS:Combined analysis of IgH and TCR gene rearragnements by the PCR technique followed by heteroduplex analysis can be a useful diagnostic adjunct to determine the clonality of various lymphoproliferative diseases with high sensitivity. But clinical, morphological and immunophenotypical correlation should be considered to reach the final diagnosis due to a few false positive cases.
