Proliferation Patterns of N-butyl-N- ( 4-hydroxybutyl ) Nitrosamine ( BBN ) Induced Rat Bladder Tumor Identified by Histone mRNA In Situ Hybridization.
- Author:
Chang Ou RYU
1
;
Jung Won SEO
;
Yong Tae KIM
;
Won Jae KIM
Author Information
1. Chungbuk National University, Cheongju, Korea.
- Publication Type:Original Article
- Keywords:
BBN;
bladder tumor;
histone mRNA
- MeSH:
Animals;
Carcinogenesis;
Cell Cycle;
Cytoplasm;
DNA Replication;
Histones*;
Hyperplasia;
In Situ Hybridization*;
Rats*;
RNA, Messenger*;
Urinary Bladder Neoplasms*;
Urinary Bladder*
- From:Korean Journal of Urology
1997;38(2):111-119
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The synthesis of histone mRNA is closely coupled with DNA replication. During the S-phase of the cell cycle, the level of histone mRNA increases over fifty fold then rapidly disappears at the start of G2-phase. The presence, therefore, of abundant quantities of histone mRNA provides a molecular marker of cycling cells. The expression of histone mRNA was investigated by in situ hybridization method in N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) induced rat bladder tumor to determine the proliferation patterns. This method resulted in intense brown/black cytoplasmic staining of cells containing histone mRNA and extent and intensity of stain were increased in tumorigenesis (normal, simple hyperplasia, nodular or papillary hyperplasia, and transitional cell carcinoma-Ta,T1). The higher S-phase fraction indicated greater biological malignancy based on the fact that the extent and intensity of stain progressively increased with tumorigenesis.
