Controlled release of nerve growth factor from heparin-conjugated fibrin gel within the nerve growth factor-delivering implant.
10.5125/jkaoms.2014.40.1.3
- Author:
Jin Yong LEE
1
;
Soung Min KIM
;
Myung Jin KIM
;
Jong Ho LEE
Author Information
1. Department of Oral and Maxillofacial Surgery, Korea University Guro Hospital, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Nerve growth factor-delivering implant;
Nerve growth factor;
Controlled release;
Nerve regeneration
- MeSH:
Animals;
Dental Implants;
Enzyme-Linked Immunosorbent Assay;
Fibrin*;
Fibrinogen;
Half-Life;
Nerve Growth Factor*;
Nerve Regeneration;
Neurites;
PC12 Cells;
Peripheral Nerves;
Pheochromocytoma;
Regeneration;
Thrombin
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2014;40(1):3-10
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: Although nerve growth factor (NGF) could promote the functional regeneration of an injured peripheral nerve, it is very difficult for NGF to sustain the therapeutic dose in the defect due to its short half-life. In this study, we loaded the NGF-bound heparin-conjugated fibrin (HCF) gel in the NGF-delivering implants and analyzed the time-dependent release of NGF and its bioactivity to evaluate the clinical effectiveness. MATERIALS AND METHODS: NGF solution was made of 1.0 mg of NGF and 1.0 mL of phosphate buffered saline (PBS). Experimental group A consisted of three implants, in which 0.25 microL of NGF solution, 0.75 microL of HCF, 1.0 microL of fibrinogen and 2.0 microL of thrombin was injected via apex hole with micropipette and gelated, were put into the centrifuge tube. Three implants of experimental group B were prepared with the mixture of 0.5 microL of NGF solution, 0.5 microL HCF, 1.0 microL of fibrinogen and 2.0 microL of thrombin. These six centrifuge tubes were filled with 1.0 mL of PBS and stirred in the water-filled beaker at 50 rpm. At 1, 3, 5, 7, 10, and 14 days, 1.0 mL of solution in each tubes was collected and preserved at -20degrees C with adding same amount of fresh PBS. Enzyme-linked immunosorbent assay (ELISA) was done to determine in vitro release profile of NGF and its bioactivity was evaluated with neural differentiation of pheochromocytoma (PC12) cells. RESULTS: The average concentration of released NGF in the group A and B increased for the first 5 days and then gradually decreased. Almost all of NGF was released during 10 days. Released NGF from two groups could promote neural differentiation and neurite outgrowth of PC12 cells and these bioactivity was maintained over 14 days. CONCLUSION: Controlled release system using NGF-HCF gel via NGF-delivering implant could be an another vehicle of delivering NGF to promote the nerve regeneration of dental implant related nerve damage.