Development of a Swine Benign Biliary Stricture Model Using Endoscopic Biliary Radiofrequency Ablation.
10.3346/jkms.2016.31.9.1438
- Author:
Jin Seok PARK
1
;
Seok JEONG
;
Joon Mee KIM
;
Sang Soon PARK
;
Don Haeng LEE
Author Information
1. Digestive Disease Center, Department of Internal Medicine, Inha University School of Medicine, Incheon, Korea.
- Publication Type:Original Article
- Keywords:
Benign Stricture;
Radiofrequency Ablation;
Animal Experimentation;
Endoscopes;
Safety
- MeSH:
Animal Experimentation;
Animals;
Bile Ducts;
Catheter Ablation*;
Catheters;
Cholangiography;
Common Bile Duct;
Constriction, Pathologic*;
Electrodes;
Endoscopes;
Fibrosis;
Follow-Up Studies;
Inflammation;
Models, Animal;
Phenobarbital;
Swine*
- From:Journal of Korean Medical Science
2016;31(9):1438-1444
- CountryRepublic of Korea
- Language:English
-
Abstract:
The large animal model with benign biliary stricture (BBS) is essential to undergo experiment on developing new devices and endoscopic treatment. This study conducted to establish a clinically relevant porcine BBS model by means of endobiliary radiofrequency ablation (RFA). Endoscopic retrograde cholangiography (ERC) was performed on 12 swine. The animals were allocated to three groups (60, 80, and 100 W) according to the electrical power level of RFA electrode. Endobiliary RFA was applied to the common bile duct for 60 seconds using an RFA catheter that was endoscopically inserted. ERC was repeated two and four weeks, respectively, after the RFA to identify BBS. After the strictures were identified, histologic evaluations were performed. On the follow-up ERC two weeks after the procedure, a segmental bile duct stricture was observed in all animals. On microscopic examination, severe periductal fibrosis and luminal obliteration with transmural inflammation were demonstrated. Bile duct perforations occurred in two pigs (100 W, n = 1; 80 W, n = 1) but there were no major complications in the 60 W group. The application of endobiliary RFA with 60 W electrical power resulted in a safe and reproducible swine model of BBS.
