In Vitro IL-1, TNF and IL-6 Production of Peripheral Blood Mononuclear Cells Stimulated with Different Hemodiaysis Membranes.
- Author:
Yeong Hoon KIM
1
;
Dae Geon LIM
;
Hyun Dae CHO
;
Yang Wook KIM
;
Won Do PARK
;
Jae Hyung AHN
;
Tae Won LEE
;
Chun Gyoo IHM
;
Myung Jae KIM
Author Information
1. Department of Internal Medicine, Inje University College of Medicine, Pusan, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
IL-1;
IL-6;
TNF alpha;
PMMA;
Cuprophan
- MeSH:
Cytokines;
Dialysis;
Humans;
Interleukin-1*;
Interleukin-6*;
Membranes*;
Polymethyl Methacrylate;
Renal Dialysis
- From:Korean Journal of Medicine
1997;52(6):814-822
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: In order to evaluate the role of these cytokines in biological response induced by blood interaction with hemodialysis membranes. METHODS: We have investigated the IL-1, TNF and IL-6 concentrations in the supernatant of 24-hours cultured peripheral blood mononuclear cells (PBMC) without(spontaneous group) or with broken cuprophan or P1VMA membranes in 9 chronic hemodialyzed patients and 8 healthy controls. The blood samples were drawn before dialysis using following criteria: (a) in last dialytic treatment with PMMA membranes(HDEl), (b) after two weeks of dialytic treatment wih cuprophan membranes(HDE2). RESULTS: In the both of patient group(HDE1 and HDE2) and controls production of IL-l, TNF and IL-6 of PBMC stimulated with cuprophan or PMMA membrane particles was increased compared to those of spontaneous group. IL-1 production of HDE1 stimulated PMMA(99.31 +/- 30.15fmol/ml) was significantly higher compared to that of cuprophan(48.43 +/- 11.29fmol/ml), TNF production of HDE2 with cuprophan(114.86 +/- 38.5lfmoVml) was significantly high compared to that of spontaneous group(52.42 +/- 29.94fmol/ml). IL-6 production of HDE2(646.70 +/- 103.84fmol/ml) was significantly high compared to that of spontaneous group(385.88 +/- 87.03fmoVml). Comparing cytokine production of PBMC, there was a significant correlation between IL-1 and IL-6(r=0.78), IL-1 and TNF(r=0.78) and TNF and IL-6(r=0,76). CONCLUSION: Our results show that the interaction of cuprophan or PMMA membranes with blood increase the production of IL-1, TNF and IL-6. We suggest that in patients undergoing routine hemodialysis PBMC are primed by exposure to chronic stimulation.
