Dissemination of Plasmid-mediated qnr, aac(6')-Ib-cr, and qepA Genes Among 16S rRNA Methylase Producing Enterobacteriaceae in Korea.
10.4167/jbv.2009.39.3.173
- Author:
Hee Young KANG
1
;
Migma Dorji TAMANG
;
Sung Yong SEOL
;
Jungmin KIM
Author Information
1. Department of Microbiology, School of Medicine, Kyungpook National University, Daegu, Korea. minkim@knu.ac.kr
- Publication Type:Original Article
- Keywords:
qnr;
aac(6')-Ib-cr;
qepA;
armA;
rmtB;
Resistance
- MeSH:
Chimera;
Cloaca;
Enterobacteriaceae;
Humans;
Korea;
Methyltransferases;
Plasmids;
Pneumonia;
Replicon
- From:Journal of Bacteriology and Virology
2009;39(3):173-182
- CountryRepublic of Korea
- Language:English
-
Abstract:
Plasmid-mediated quinolone resistance (PMQR) genes: qnr, aac(6')-Ib-cr, and qepA were investigated among 153 armA and 51 rmtB-positive transconjugants and their 204 clinical isolates of Enterobacteriaceae. Overall, qnrB4 and aac(6')-Ib-cr genes were identified in 52.3% (63 K. pneumoniae, 10 E. coli, 4 E. cloacae, and 3 E. aerogenes) and 24.8% (16 K. pneumoniae, 8 E. coli, 6 S. marcescens, 4 E. cloacae, 3 C. freundii and 1 K. oxytoca) of 153 armA-positive isolates, respectively. Four isolates of K. pneumoniae and two isolates of E. coli positive for armA co-harbored both qnrB4 and aac(6')-Ib-cr. The qepA gene was detected in 11.8% (5 E. coli and 1 K. pneumoniae) of 51 rmtB-positive clinical isolates and their transconjugants. Southern hybridization confirmed the co-localization of qepA and rmtB on a large conjugative plasmid of size between 90 to 170 kb. Inc replicon typing showed that qnrB4/6, aac(6')-Ib-cr, and qepA genes were principally disseminated by IncFIIAs, IncL/M, and IncF plasmids, respectively. This study constitutes the first report of the three known PMQR genes among the 16S rRNA methylase producing Enterobacteriaceae isolates of human origin from Korea.
