Effects of Interferon Gamma on Collagen Expression in the Partially Obstructed Urinary Bladder of Rat.
- Author:
Kyu Sung LEE
1
;
Kwang Myung KIM
;
Jung Yun JUNG
;
Ahnkie LEE
;
Hae Il CHEONG
;
Cheol Keun PARK
;
Shi Whang KIM
Author Information
1. Department of Urology, Sungkyunkwan University School of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Urinary bladder;
Collagen;
Interferon-gamma;
Bladder outlet obstruction
- MeSH:
Animals;
Antibodies;
Collagen Type I;
Collagen Type III;
Collagen*;
Compliance;
Down-Regulation;
Fibroblasts;
Gene Expression;
Humans;
Injections, Subcutaneous;
Interferon-gamma;
Interferons*;
Mucous Membrane;
Myofibroblasts;
Rats*;
RNA, Messenger;
Up-Regulation;
Urinary Bladder Neck Obstruction;
Urinary Bladder*
- From:Korean Journal of Urology
1999;40(6):740-748
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Recent studies have proved that bladder compliance is closely related to the collagen content of the bladder. It has been shown through human fibroblast, condrocyte, and rat myofibroblasts that interferon(IFN)-gammadecreased collagen synthesis. The objective of this study was to investigate the alteration of collagen deposition and gene expressions in collagen types I and III by partial bladder outlet obstruction and to compare the changes during IFN-gamma treatment. MATERIALS AND METHODS: Following 4 weeks of partial bladder outlet obstruction in 200-250gm. SD rats, the bladder tissues were evaluated for collagen deposition by an immunohistochemical staining using the polyclonal antibodies and the messenger ribonucleic acid(mRNA) contents of the collagen type I and III were evaluated by Northern hybridization. Recombinant IFN-gamma(100,000 units) was administered once a day for 4 weeks by subcutaneous injection. RESULTS: In the control bladders, the immunohistochemical staining for type I and type III collagen protein showed an intense localization in the lamina propria with modest localization in the inter-fascicular region. With obstruction type I and type III collagen staining intensified, especially in the inter-fascicular region. The IFN-gamma administration reduced the inter-fascicular deposition of collagen type I and III. There was an up-regulation of collagen type I and III gene expression after the obstruction. In the obstructed bladders proalpha1(I) and proalpha1(III) collagen mRNA intensity was increased 3.4 and 5.1 folds than the control groups, respectively. This up-regulation was down-regulated by IFN-gamma administration. CONCLUSIONS: The down regulation of collagen types I and III gene expression, and decreased protein deposition after IFN-gamma treatment demonstrated that the effect of IFN-gamma was, in part, transcriptionally regulated. These results suggest that the recombinant IFN-gamma may be beneficial in the treatment of patients with poorly compliant bladder.
