Additive Estrogenic Activities of the Binary Mixtures of Four Estrogenic Chemicals in Recombinant Yeast Expressing Human Estrogen Receptor.
- Author:
Kyung Sun KANG
1
;
Sung Dae CHO
;
Yong Soon LEE
Author Information
1. Department of Veterinary Public Health, College of Veterinary Medicine, School of Agricultural Biotechnology, Seoul National University, Seodun-Dong, Kwonsun-Ku, Suwon 441-744, Korea. leeys@snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Recombinant yeast;
human estrogen receptor;
beta-galactosidase;
combination;
additive
- MeSH:
Cloning, Molecular;
Estradiol/pharmacology;
Estrogens/classification/*pharmacology;
Estrogens, Non-Steroidal/*pharmacology;
Humans;
Kinetics;
Parabens/pharmacology;
Phenols/pharmacology;
Receptors, Estrogen/drug effects/*physiology;
Recombinant Proteins/drug effects/metabolism;
Saccharomyces cerevisiae/genetics
- From:Journal of Veterinary Science
2002;3(1):1-5
- CountryRepublic of Korea
- Language:English
-
Abstract:
To evaluate the estrogenic activities of several chemicals such as 17 beta-estradiol (E2), rho-nonylphenol, bisphenol A, butylparaben, and combinations of these chemicals, we used recombinant yeasts containing the human estrogen receptor [Saccharomyces cerevisiae ER + LYS 8127]. We evaluated E2 was most active in the recombinant yeast assay, followed by rho-nonylphenol, bisphenol A, butylparaben. The combinations of some concentrations of 17-estradiol as a strong estrogen and bisphenol A or butylparaben as a weak estrogen showed additive estrogenic effects. Also, the combinations of some concentrations of nonlyphenol and butylparaben and combination of butylparaben and bisphenol A showed additive effects in the estrogenic activity. Therefore, the estrogenic activities of the combinations of two chemicals were additive, not synergistic.
