Detection of p53 Gene Mutations in Gastric cancers: Polymerase Chain Reaction and Single Strand Conformational Polymorphism Migration Technique.
- Author:
Young Jin KIM
1
;
Ji Yun KOOK
;
Shin Kon KIM
;
Soon Pal SUH
;
Jin Pok KIM
Author Information
1. Department of Surgery, Chonnam National University Medical College, Korea.
- Publication Type:Original Article
- Keywords:
Gastric cancer;
p53;
PCR-SSCP
- MeSH:
Adenocarcinoma;
Animals;
Exons;
Genes, p53*;
Humans;
Mice;
Oncogenes;
Polymerase Chain Reaction*;
Proliferating Cell Nuclear Antigen;
Stomach Neoplasms*
- From:Journal of the Korean Surgical Society
1997;52(6):839-845
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Abberations of the p53 gene in 24 primary gastric carcinomas were examined by single-strand conformation polymorphism analysis of polymerase chain reaction products and by immunohistochemical staining with monoclonal antibody. Of these gastric adenocarcinomas, 23 were advanced gastric carcinomas, and 1 was early gastric cancer. Using polymerase chain reaction (PCR) and a single-strand conformation polymorphism migration technique (SSCP), the presence of mutations in exons 4-9 was evaluated. Using the mouse specific anti-human p53 monoclonal antibody, we also looked for overexpression of the p53 protein in tissue sections. In 5 cases shifted bands were reproducibly identified by PCR-SSCP, and all mutations were in exon 4 and 5,6. Thirteen of the tumor samples were positively stained with the monoclonal antibody. In only one of the 5 mutated cases a positive immunostaining was observed, and we couldn't find the any correlation between the mutations detected by PCR-SSCP and immunostaining. The presence of a p53 mutation by PCR-SSCP was associated with lower PCNA labeling index ( mean = 40.8:75.8%). In the cases with overexpression of p53 protein, the pathologic stages were more advanced than without overexpression of p53 protein (p=0.037). The mutation of p53 was not correlated with mutations of other oncogenes such as c-myc and c-Ha ras. Our results in this group of patients suggest that p53 mutations detected by PCR-SSCP should be reevaluated, and p53 mutations detected by immunostaining was more reliable.
