The effects of irradiation on the mRNA expression of type I collagen and alkaline phosphatase in the MC3T3-E1 osteoblastic cell line.
- Author:
Sun Young CHOI
1
;
Kwang Joon KOH
Author Information
1. Department of Oral & Maxillofacial Radiology, School of Dentistry, Chonbuk National University, Korea. radkoh@moak.chonbuk.ac.kr
- Publication Type:Original Article
- Keywords:
irradiation;
collagen type I;
alkaline phosphatase;
osteoblasts
- MeSH:
Alkaline Phosphatase*;
Cell Line*;
Cesium;
Collagen Type I*;
DNA, Complementary;
Osteoblasts*;
Polymerase Chain Reaction;
RNA;
RNA, Messenger*
- From:Korean Journal of Oral and Maxillofacial Radiology
2003;33(1):51-57
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effects of irradiation on the phenotypic expression of the MC3T3-E1 osteoblastic cell line, particularly an the expression of type I collagen and alkaline phosphatase mRNA. MATERIALS AND METHODS: Cells were irradiated with a single dose of 0.5, 1, 2, 4, and 8 Gy at a dose rate of 5.38 Gy/min using a cesium 137 irradiator. The specimens were then harvested and RNA extraction was carried out at 1 and 3 days after irradiation. The extracted RNA strands were reverse-transcribed and the resulting cDNA fragments were amplified by PCR. RESULTS: The irradiated cells demonstrated a dose-dependent increase in type I collagen mRNA expression relative to the control group, with a maximum level of type I collagen mRNA expression occurring at 8 Gy. The degree of type I collagen mRNA expression increased significantly at 1 day after irradiation, but little differences were found between the control group and at the 3rd day. The amount of alkaline phosphatase mRNA expression increased significantly at 1 and 3 days after irradiation in the 1 Gy exposed group compared with the control group. CONCLUSION: The amount of type I collagen and alkaline phosphatase mRNA expression increased significantly 1 day after irradiation when compared with the control group.
