Development and validation of a UPLC-MS/MS method for the quantification of acetaminophen in human plasma and its application to pharmacokinetic studies.
10.12793/tcp.2016.24.1.30
- Author:
Jaemin CHA
1
;
Bo Kyung KIM
;
Mi Ri GWON
;
Joomi LEE
;
Boram OHK
;
Woo Youl KANG
;
Mi sun LIM
;
Sook Jin SEONG
;
Hyun Ju KIM
;
Hae Won LEE
;
Young Ran YOON
Author Information
1. Department of Biomedical Science, BK21 Plus KNU Bio-Medical Convergence Program for Creative Talent and Clinical Trial Center, Kyungpook National University Graduate School and Hospital, Daegu 41944, Korea. yry@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Acetaminophen;
Human plasma;
UPLC-MS/MS;
Validation;
Pharmacokinetic study
- MeSH:
Acetaminophen*;
Administration, Oral;
Healthy Volunteers;
Humans*;
Ions;
Mass Spectrometry;
Phenacetin;
Plasma*
- From:Translational and Clinical Pharmacology
2016;24(1):30-36
- CountryRepublic of Korea
- Language:English
-
Abstract:
We developed an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the determination of acetaminophen concentration in human plasma. Following protein precipitated extraction, the analytes were separated and analyzed using an UPLC-MS/MS in the multiple reaction monitoring (MRM) mode with the respective [M+H]+ ions, m/z 152.06 → 110.16 for acetaminophen and m/z 180.18 → 138.12 for phenacetin (internal standard, IS). The method showed a linear response from 1 to 100 µg/mL (r > 0.9982). The limit of quantitation for acetaminophen in plasma was 1 µg/mL. The intra- and inter-day accuracy ranged in the ranges of 94.40–99.56% and 90.00–99.20%, respectively. The intra- and inter-day precision ranged in the ranges of 2.64–10.76% and 6.84–15.83%, respectively. This method was simple, reliable, precise and accurate and can be used to determine the concentration of acetaminophen in human plasma. Finally, this fully validated method was successfully applied to a pharmacokinetic study of acetaminophen in healthy volunteers following oral administration.
