Enhancement of Protein Productivity of Recombinant Hepatitis A Virus VP1 in Stably Transfected Drosophila melanogaster S2 Cells.
- Author:
Hwang Bo JEON
1
;
Jong Hwa PARK
;
Hyun Ho LEE
;
Do Hyung KIM
;
Hee Young LEE
;
Dong Hwa SHON
;
Wonyong KIM
;
In Sik CHUNG
Author Information
- Publication Type:Original Article
- Keywords: Drosophila melanogaster S2 cells; Hepatitis A virus (HAV) VP1; Dimethysulfoxide (DMSO); Sodium butyrate; Spinner flask
- MeSH: Butyrates; Capsid Proteins; Dimethyl Sulfoxide; Drosophila; Drosophila melanogaster; Efficiency; Hepatitis; Hepatitis A; Hepatitis A virus; Sodium
- From:Journal of Bacteriology and Virology 2012;42(1):69-75
- CountryRepublic of Korea
- Language:English
- Abstract: The effect of DMSO and sodium butyrate on the production of recombinant hepatitis A virus (HAV) capsid protein VP1 was evaluated and optimized in the culture of stably transfected Drosophila melanogaster S2 cells using culture plates and spinner flasks. The effect of DMSO and sodium butyrate was also evaluated to improve the recombinant VP1 production in stably transfected Drosophila S2 cells. A production level of 0.88 mg of recombinant VP1/liter was obtained in the culture-plate culture of stably transfected S2 cells at 6 days after induction with 0.5 mM CuSO4. The supplements of 2% DMSO and 10 mM sodium butyrate at 4 days post-inoculation increased recombinant VP1 accumulation by 141 and 104%, respectively, resulting in 2.17 and 1.7 mg/liter of recombinant VP1 production. In spinner flasks, recombinant VP1 production reached maximum level at 9 days after induction with 0.5 mM CuSO4, with approximately 4.96 mg/liter of recombinant VP1 production level. When 2% DMSO or 10 mM sodium butyrate was added at 5 days post-inoculation, the recombinant VP1 production was increased to 8.35 and 5.85 mg/liter, respectively. However, the synergistic effects of DMSO and sodium butyrate were not observed. These results indicate that DMSO and/or sodium butyrate can be successfully used to improve the recombinant HAV VP1 production in culture plates and spinner flasks.
