Anti-inflammatory Activity of Carpinus tschonoskii Leaves Extract in R848-stimulated Bone Marrow-derived Macrophages and Dendritic Cells.
- Author:
Sung Ha KANG
1
;
Jung Eun KOO
;
Hye Jin HONG
;
Vivek Bhakta MATHEMA
;
Young Sang KOH
Author Information
- Publication Type:Original Article
- Keywords: Carpinus tschonoskii; Pro-inflammatory cytokine; NF-kappaB; TLR7
- MeSH: Betulaceae; Cell Line; Corynebacterium; Dendritic Cells; Genes, Reporter; Humans; Interleukin-12; Interleukin-6; Kidney; Luciferases; Macrophages; Methanol; NF-kappa B
- From:Journal of Bacteriology and Virology 2012;42(1):77-82
- CountryRepublic of Korea
- Language:English
- Abstract: The present study aims to evaluate the anti-inflammatory effect of methanol extract from leaves of Carpinus tschonoskii (CE) on R848-stimulated primary bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs). Primary BMDMs and BMDCs were used for pro-inflammatory cytokine production. Human embryonic kidney cell line 293T (HEK293T) was used to access NF-kappaB activity. In all cases, R848 was used to stimulate the cells. The CE (0~150 microg/ml) was treated to BMDMs, BMDCs, and HEK293T cells. CE pre-treatment in R848-stimulated BMDMs and BMDCs showed a dose-dependent inhibitory effect on pro-inflammatory cytokine (e.g., IL-12 p40, IL-6, and TNF-alpha) production as compared to non-treated controls. In NF-kappaB reporter gene assay, the CE pre-treatment inhibited NF-kappaB-dependent luciferase activity in a dose-dependent manner. Overall, our findings suggest that CE has significant inhibitory effect on pro-inflammatory cytokine production and deserve further studies concerning potentials of CE for medicinal uses.
