Gingko biloba extract (EGb 761) attenuates ischemic brain injury-induced reduction in Ca2+ sensor protein hippocalcin.
10.5625/lar.2012.28.3.199
- Author:
Phil Ok KOH
1
Author Information
1. Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Sciences, Gyeongsang National University, Jinju, Korea. pokoh@gnu.ac.kr
- Publication Type:Letter
- Keywords:
EGb 761;
hippocalcin;
neuroprotection
- MeSH:
Animals;
Blotting, Western;
Brain;
Brain Injuries;
Brain Ischemia;
Calcium;
Cell Death;
Cerebral Cortex;
Ginkgo biloba;
Hippocalcin;
Humans;
Infarction, Middle Cerebral Artery;
Male;
Neurons;
Plant Extracts;
Rats, Sprague-Dawley
- From:Laboratory Animal Research
2012;28(3):199-204
- CountryRepublic of Korea
- Language:English
-
Abstract:
Gingko biloba extract 761 (EGb 761) protects neuronal cells from ischemic brain injury via a number of neuroprotective mechanisms. Hippocalcin is a calcium sensor protein that regulates intracellular calcium concentrations and apoptotic cell death. We investigated whether EGb 761 regulates hippocalcin expression in cerebral ischemia. Male Sprague-Dawley rats were treated with vehicle or EGb 761 (100 mg/kg) prior to middle cerebral artery occlusion (MCAO), and cerebral cortex tissues were collected 24 h after MCAO. A proteomic approach demonstrated reduction in hippocalcin expression in vehicle-treated animals during MCAO, whereas EGb 761 treatment prevented injury-induced decreases in hippocalcin expression. RT-PCR and Western blot analyses indicated that EGb 761 attenuates injury-induced decrease in hippocalcin. These results suggest that the maintenance of hippocalcin during cerebral ischemia contributes to the neuroprotective role of EGb 761.
