Evaluation of New Third Generation LG Anti-HIV 1/2 Plus ELISA Kit.
- Author:
Joon Sup YEOM
1
;
Seung Ho RYU
;
Hee Jung KANG
;
Jae Won PARK
Author Information
1. Department of Internal Medicine, Sungkyunkwan University School of Medicine.
- Publication Type:Original Article
- Keywords:
Anti-HIV;
Enzyme-linked immunosorbent assay;
Sensitivity and specificity
- MeSH:
Alcoholics;
Biological Science Disciplines;
Enzyme-Linked Immunosorbent Assay*;
Hepatitis B Surface Antigens;
HIV;
HIV-1;
HIV-2;
Humans;
Kidney Failure, Chronic;
Mass Screening;
Sensitivity and Specificity;
Seoul;
Tissue Donors
- From:Korean Journal of Blood Transfusion
2003;14(2):153-159
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: In several studies, failure of some of the previously developed anti-HIV-1/HIV-2 ELISA tests to detect HIV-1 group O infected blood had been reported. All of the anti-HIV-1/HIV-2 ELISA tests made by Korean manufactures have limitation in detecting HIV-1 group O infected blood. Newly developed LG Anti-HIV 1/2 Plus is the first Korean made product to overcome this limitation. METHODS: Sensitivity and specificity of LG Anti-HIV 1/2 Plus (LG Life Science, Seoul, Korea) and Enzygnost Anti-HIV 1/2 Plus (Dade Behring, Marburg, Germany) were evaluated using 82 Anti-HIV 1 positive samples, 2 Anti-HIV 1 group O positive samples, 25 Anti-HIV 2 positive samples and commercial BBI (Boston Biomedica, Inc., MA, USA)) Anti-HIV panels and 382 Anti HIV negative samples (300 healthy donors, 13 alcoholics sera, 20 end-stage renal disease sera, 20 HBsAg positive sera, 22 anti-HBs antibody positive sera, 7 anti-HCV positive sera) respectively. Seroconversion window was evaluated using 2 BBI(R) seroconversion panel. Intrapersonal and interpersonal reproducibility of the test were evaluated also. RESULTS: Sensitivity and specificity of both tests were 100%. Analysis of window period using commercial seroconversion panel showed no difference between both tests. CONCLUSION: LG Anti-HIV 1/2 Plus LISA showed excellent sensitivity and specificity in tested samples including HIV-1 group O infected sera. It can be concluded that LG Anti-HIV 1/2 Plus ELISA is capable of detecting most of the HIV-1, HIV-2 type and it could be used in screening donated blood.
