Regulation of type I collagen and interstitial collagenase mRNA expression in human dermal fibroblasts by colchicine and D-penicillamine.
10.3349/ymj.1999.40.5.490
- Author:
Kee Yang CHUNG
1
;
Dong Seung KANG
Author Information
1. Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea. kychung@yumc.yonsei.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Sclerosis;
type I collagen;
interstitial collagenase;
mRNA;
TGF-beta;
colchicine;
D-penicillamine
- MeSH:
Cells, Cultured;
Colchicine/pharmacology*;
Collagen/genetics*;
Fibroblasts/metabolism;
Gene Expression Regulation/drug effects*;
Human;
Interstitial Collagenase/genetics*;
Penicillamine/pharmacology*;
RNA, Messenger/analysis*;
Skin/metabolism;
Skin/cytology;
Transforming Growth Factor beta/pharmacology
- From:Yonsei Medical Journal
1999;40(5):490-495
- CountryRepublic of Korea
- Language:English
-
Abstract:
Sclerosis is a disease process in which idiopathic hardening occurs in the skin and/or internal organs as a result of the accumulation of type I collagen, induced mainly by transforming growth factor-beta. Colchicine and D-penicillamine are widely used for its treatment. Their effects are known to be due to post-translational down-regulation of type I collagen synthesis, with colchicine also up-regulating interstitial collagenase. To determine whether or not they have any pre-translational effect on type I collagen and MMP-1, and also to observe their effects on the action of TGF-beta, cultured neonatal foreskin fibroblasts were treated with colchicine and D-penicillamine, singly and together. The amount of type I collagen and MMP-1 mRNA were quantitated by Northern blot hybridization. Colchicine suppresses the basal level of type I collagen mRNA but minimally stimulates the mRNA expression of MMP-1, whereas D-penicillamine does not have any significant effects on either. Colchicine was also able to significantly suppress the TGF-beta-induced up-regulation of type I collagen mRNA expression.
