Neuroprotective Effect of Anthocyanin on Experimental Traumatic Spinal Cord Injury.
10.3340/jkns.2011.49.4.205
- Author:
Kyoung Tae KIM
1
;
Taek Kyun NAM
;
Yong Sook PARK
;
Young Baeg KIM
;
Seung Won PARK
Author Information
1. Department of Neurosurgery, Kyungpook National University Hospital, Daegu, Korea.
- Publication Type:Original Article
- Keywords:
Spinal cord trauma;
Anthocyanin;
Antioxidants
- MeSH:
Administration, Oral;
Animals;
Anthocyanins;
Antioxidants;
Cell Count;
Fluorescence;
Foot;
Horns;
Intubation;
Motor Neurons;
Neurons;
Neuroprotective Agents;
Oxygen;
Spinal Cord;
Spinal Cord Injuries;
Stomach;
Superoxides
- From:Journal of Korean Neurosurgical Society
2011;49(4):205-211
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: We investigated the neuroprotective effect of anthocyanin, oxygen radical scavenger extracted from raspberries, after traumatic spinal cord injury (SCI) in rats. METHODS: The animals were divided into two groups : the vehicle-treated group (control group, n=20) received an oral administration of normal saline via stomach intubation immediately after SCI, and the anthocyanin-treated group (AT group, n=20) received 400 mg/kg of cyanidin 3-O-beta-glucoside (C3G) in the same way. We compared the neurological functions, superoxide expressions and lesion volumes in two groups. RESULTS: At 14 days after SCI, the AT group showed significant improvement of the BBB score by 16.7+/-3.4%, platform hang by 40.0+/-9.1% and hind foot bar grab by 30.8+/-8.4% (p<0.05 in all outcomes). The degree of superoxide expression, represented by the ratio of red fluorescence intensity, was significantly lower in the AT group (0.98+/-0.38) than the control group (1.34+/-0.24) (p<0.05). The lesion volume in lesion periphery was 32.1+/-2.4 microL in the control and 24.5+/-2.3 microL in the AT group, respectively (p<0.05), and the motor neuron cell number of the anterior horn in lesion periphery was 8.3+/-5.1 cells/HPF in the control and 13.4+/-6.3 cells/HPF in the AT group, respectively (p<0.05). CONCLUSION: Anthocyanin seemed to reduce lesion volume and neuronal loss by its antioxidant effect and these resulted in improved functional recovery.
