Detection of Chlamydia Trachomatis in Nongonococcal Urethritis by Polymerase Chain Reaction.
- Author:
Yangil PARK
1
;
Samryong LEE
;
Jongsuk OH
Author Information
1. Department of Urology, Chonnam University, Medical School, Kwangju, Korea.
- Publication Type:Original Article
- Keywords:
nongonococcal urethritis;
Chlamydia trachomatis
- MeSH:
Cell Culture Techniques;
Chlamydia trachomatis*;
Chlamydia*;
Enzyme-Linked Immunosorbent Assay;
Humans;
Polymerase Chain Reaction*;
Urethritis*;
Urination
- From:Korean Journal of Urology
1996;37(2):197-202
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Chlamydia trachomatis (C. trachomatis) is the most important pathogen of nongonococcal urethritis. C. trachomatis was detected by cell culture, enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) in 145 patients with nongonococcal urethritis. In 5 of 63 antibiotic-treated patients, C. trachomatis was identified by at least one method. C. trachomutid was identified in 34 (41.5%) of 82 nontreated patients. The most common symptom of the 34 patients was painful urination. C. trachomafir was identified in 6 cases (17.6%) of 34 patients by cell culture. And C. trachorrdtis was identified in 9 cases (26.5%) by ELISA, while in 33 cases(97.1%) by PCR. When PCR was performed with urines and urinary swabs collected from 38 patients with nongonococcal urethritis, 11(29%) cases showed positive with urine and 10(26%) cases with urinary swab. These results suggested that PCR with urine showed the higher positive detection rate of C. trachomatis in the patient with nongonococcal urethritis than other methods.
