Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca).
10.4142/jvs.2009.10.3.261
- Author:
Feng Shan GAO
1
;
Gui Xue HU
;
Xian zhu XIA
;
Yu Wei GAO
;
Ya Duo BAI
;
Xiao Huan ZOU
Author Information
1. Department of Biochemistry and Molecular Biology, College of Bioengineering, Dalian University, Dalian, Liaoning 116622, China.
- Publication Type:Brief Communication ; Case Reports
- Keywords:
canine coronavirus;
giant panda;
virus characterization;
virus isolation
- MeSH:
Amino Acid Sequence;
Animal Diseases/*virology;
Animals;
Animals, Zoo/*virology;
Coronaviridae Infections/*veterinary/virology;
Coronavirus, Canine/genetics/*isolation & purification;
Fatal Outcome;
Female;
Male;
Molecular Sequence Data;
Sequence Alignment;
Sequence Homology, Amino Acid;
Ursidae/*virology;
Viral Proteins/chemistry
- From:Journal of Veterinary Science
2009;10(3):261-263
- CountryRepublic of Korea
- Language:English
-
Abstract:
Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo. The clinical disease profile suggested that the pandas may have suffered a viral infection. Therefore, a series of detection including virus isolation, electron microscopy, cytobiological assay, serum neutralization and RT-PCR were used to identify the virus. It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV. The results suggest that the affected pandas had been infected with CCV.
