Ascitic Fluid Calprotectin and Serum Procalcitonin as Accurate Diagnostic Markers for Spontaneous Bacterial Peritonitis.
- Author:
Ahmed ABDEL-RAZIK
1
;
Nasser MOUSA
;
Dina ELHAMMADY
;
Rania ELHELALY
;
Rasha ELZEHERY
;
Sherif ELBAZ
;
Mohamed EISSA
;
Niveen EL-WAKEEL
;
Waleed ELDARS
Author Information
- Publication Type:Original Article
- Keywords: Calprotectin; Interleukin-6; Procalcitonin; Spontaneous bacterial peritonitis; Tumor necrosis factor-alpha
- MeSH: Ascites; Ascitic Fluid*; Diagnosis; Enzyme-Linked Immunosorbent Assay; Humans; Interleukin-6; Leukocyte L1 Antigen Complex*; Neutrophils; Peritonitis*; Sensitivity and Specificity; Tumor Necrosis Factor-alpha
- From:Gut and Liver 2016;10(4):624-631
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND/AIMS: The diagnosis of spontaneous bacterial peritonitis (SBP) is based on a polymorphonuclear leukocytes (PMNs) exceeding 250/μL in ascitic fluid. The aim of the study was to evaluate serum procalcitonin and ascitic fluid calprotectin as accurate diagnostic markers for detecting SBP. METHODS: Seventy-nine patients with cirrhotic ascites were included. They were divided into a SBP group, including 52 patients, and a non-SBP group of 27 patients. Serum procalcitonin, ascitic calprotectin, and serum and ascitic levels of tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) were measured using an enzyme-linked immunosorbent assay. RESULTS: Serum procalcitonin and ascitic calprotectin were significantly higher in SBP patients than in non-SBP patients. Significant increases in both serum and ascitic levels of TNF-α and IL-6 were observed in SBP patients versus non-SBP patients. At a cutoff value of 0.94 ng/mL, serum procalcitonin had 94.3% sensitivity and 91.8% specificity for detecting SBP. In addition, at a cutoff value of 445 ng/mL, ascitic calprotectin had 95.4% sensitivity and 85.2% specificity for detecting SBP. Both were positively correlated with ascitic fluid proteins, PMN count, TNF-α, and IL-6. CONCLUSIONS: According to our findings, determination of serum procalcitonin levels and ascitic calprotectin appears to provide satisfactory diagnostic markers for the diagnosis of SBP.
