Comparison of the yield of platelet separation between two methods: platelet rich plasma (PRP) and buffy coat (BC).
- Author:
Son Truong Nguyen
- Publication Type:Journal Article
- Keywords:
platelet
- MeSH:
Blood Platelets;
Purpura, Thrombocytopenic
- From:Journal of Vietnamese Medicine
1999;232(1):1-8
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Production of platelet concentrates (PCs) have an important role in the replacement therapy for severe thrombocytopenic conditions. PCs can be prepared by centrifugal technique which have two main methods: platelet rich plasma and buffy coat. We studied on 40 blood units (volume 450 ml, anticoagulant: CPD-A1, triple bags-type) and evaluated the yield of platelet (PLT) separation red blood cell (RBC) and white blood cell (WBC) contamination from these methods. The result show that the yield of PLT separation from PRP method is significantly higher than those from BC (82.60+ 7.20x1010, yield: 56.75% vs 50.59 + 2.97 yield: 32.4%) The WBC and RBC’s contamination in PCs from two methods are higher than the standard of Council of Europe. By sum up, follow studies will be necessary for decreasing the WBC and RPC’s contamination in PCs, the PRP method may be evaluated as a high-yield method in PCs production
