Human Urine-derived Stem Cells Seeded Surface Modified Composite Scaffold Grafts for Bladder Reconstruction in a Rat Model.
10.3346/jkms.2015.30.12.1754
- Author:
Jun Nyung LEE
1
;
So Young CHUN
;
Hyo Jung LEE
;
Yu Jin JANG
;
Seock Hwan CHOI
;
Dae Hwan KIM
;
Se Heang OH
;
Phil Hyun SONG
;
Jin Ho LEE
;
Jong Kun KIM
;
Tae Gyun KWON
Author Information
1. Department of Urology, Kyungpook National University School of Medicine, Daegu, Korea. tgkwon@knu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Bladder Regeneration;
Surface Modified Scaffold;
Urine-derived Stem Cells;
Basic Fibroblast Growth Factor 2
- MeSH:
Adult Stem Cells/cytology/metabolism/*transplantation;
Animals;
Biocompatible Materials/chemistry;
Cell Differentiation;
Fibroblast Growth Factor 2/administration & dosage;
Heparin/administration & dosage;
Humans;
Materials Testing;
Models, Animal;
Poloxamer;
Polyesters;
Rats;
Reconstructive Surgical Procedures;
Regeneration;
Tissue Engineering/*methods;
Tissue Scaffolds/chemistry;
Urinary Bladder/anatomy & histology/physiology/*surgery;
Urine/*cytology
- From:Journal of Korean Medical Science
2015;30(12):1754-1763
- CountryRepublic of Korea
- Language:English
-
Abstract:
We conducted this study to investigate the synergistic effect of human urine-derived stem cells (USCs) and surface modified composite scaffold for bladder reconstruction in a rat model. The composite scaffold (Polycaprolactone/Pluronic F127/3 wt% bladder submucosa matrix) was fabricated using an immersion precipitation method, and heparin was immobilized on the surface via covalent conjugation. Basic fibroblast growth factor (bFGF) was loaded onto the heparin-immobilized scaffold by a simple dipping method. In maximal bladder capacity and compliance analysis at 8 weeks post operation, the USCs-scaffold(heparin-bFGF) group showed significant functional improvement (2.34 ± 0.25 mL and 55.09 ± 11.81 microL/cm H2O) compared to the other groups (2.60 ± 0.23 mL and 56.14 ± 9.00 microL/cm H2O for the control group, 1.46 ± 0.18 mL and 34.27 ± 4.42 microL/cm H2O for the partial cystectomy group, 1.76 ± 0.22 mL and 35.62 ± 6.69 microL/cm H2O for the scaffold group, and 1.92 ± 0.29 mL and 40.74 ± 7.88 microL/cm H2O for the scaffold(heparin-bFGF) group, respectively). In histological and immunohistochemical analysis, the USC-scaffold(heparin-bFGF) group showed pronounced, well-differentiated, and organized smooth muscle bundle formation, a multi-layered and pan-cytokeratin-positive urothelium, and high condensation of submucosal area. The USCs seeded scaffold(heparin-bFGF) exhibits significantly increased bladder capacity, compliance, regeneration of smooth muscle tissue, multi-layered urothelium, and condensed submucosa layers at the in vivo study.
