Urinary Nucleic Acid TSPAN13-to-S100A9 Ratio as a Diagnostic Marker in Prostate Cancer.
10.3346/jkms.2015.30.12.1784
- Author:
Chunri YAN
1
;
Ye Hwan KIM
;
Ho Won KANG
;
Sung Phil SEO
;
Pildu JEONG
;
Il Seok LEE
;
Dongho KIM
;
Jung Min KIM
;
Yung Hyun CHOI
;
Sung Kwon MOON
;
Seok Joong YUN
;
Wun Jae KIM
Author Information
1. Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea. wjkim@chungbuk.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't ; Validation Studies
- Keywords:
Prostatic Neoplasms;
Cell-free Nucleic Acid;
Urine;
Two-gene Expression Ratio;
S100A9;
TSPAN13;
Diagnostic Marker
- MeSH:
Aged;
Aged, 80 and over;
Biomarkers, Tumor/*genetics/*urine;
Calgranulin B/*genetics;
Cohort Studies;
Humans;
Male;
Middle Aged;
Nucleic Acids/*genetics/*urine;
Oligonucleotide Array Sequence Analysis;
Prostate/metabolism;
Prostatic Hyperplasia/diagnosis/genetics/urine;
Prostatic Neoplasms/diagnosis/*genetics/*urine;
RNA, Messenger/genetics/metabolism;
RNA, Neoplasm/genetics/metabolism;
ROC Curve;
Real-Time Polymerase Chain Reaction;
Tetraspanins/*genetics
- From:Journal of Korean Medical Science
2015;30(12):1784-1792
- CountryRepublic of Korea
- Language:English
-
Abstract:
The potential use of urinary nucleic acids as diagnostic markers in prostate cancer (PCa) was evaluated. Ninety-five urine samples and 234 prostate tissue samples from patients with PCa and benign prostatic hyperplasia (BPH) were analyzed. Micro-array analysis was used to identify candidate genes, which were verified by the two-gene expression ratio and validated in tissue mRNA and urinary nucleic acid cohorts. Real-time quantitative polymerase chain reaction (qPCR) was used to measure urinary nucleic acid levels and tissue mRNA expression. The TSPAN13-to-S100A9 ratio was selected to determine the diagnostic value of urinary nucleic acids in PCa (P = 0.037) and shown to be significantly higher in PCa than in BPH in the mRNA and nucleic acid cohort analyses (P < 0.001 and P = 0.013, respectively). Receiver operating characteristic (ROC) analysis showed that the area under the ROC curve was 0.898 and 0.676 in tissue mRNA cohort and urinary nucleic acid cohort, respectively. The TSPAN13-to-S100A9 ratio showed a strong potential as a diagnostic marker for PCa. The present results suggest that the analysis of urine supernatant can be used as a simple diagnostic method for PCa that can be adapted to the clinical setting in the future.
