Detection of Bacillus anthracis using a nested PCR Method.
- Author:
Yong Keel CHOI
;
Seong Kun CHO
;
Myung Hee KIM
;
Seung Yun BAIK
;
Gyeong Hyun PARK
;
Young Gyu CHAI
- Publication Type:Original Article
- MeSH:
Animals;
Anthrax;
Bacillus anthracis*;
Bacillus*;
DNA;
DNA Primers;
Humans;
Plasmids;
Polymerase Chain Reaction*;
Soil
- From:Journal of the Korean Society for Microbiology
1998;33(6):583-588
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Bacillus anthracis is a soil pathogen capable of causing anthrax in animals and humans. To establish a method for specifically detecting B. anthracis, we used nested polymerase chain reaction. Outer and inner sets of oligonucleotide primers were designed from the protective antigen (pag) gene and from the cya gene of the plasmid pXO1. Ainplification of 482 bp or 208 bp DNA fragment obtained from a nested PCR method provided the basis for rapid and reliable assay for the detection and identification of B. anthracis.
