Isolation of GTP binding from bovine brain.
10.12701/yujm.1993.10.2.360
- Author:
Jung Hye KIM
- Publication Type:Original Article
- MeSH:
Brain*;
Cell Membrane;
Cholates;
Chromatography;
Electrophoresis;
GTP-Binding Proteins;
Guanosine Triphosphate*;
Membranes;
Molecular Weight;
Signal Transduction
- From:Yeungnam University Journal of Medicine
1993;10(2):360-368
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
GTP binding protein (G-protein) associated with membrane and involved in signal transduction was isolated from bovine brain, and molecular weight of G protein was observed. As the results, cell membranes were homogenized from bovine brain tissues and proteins of membrane were gained using 1% cholate, and progressed the chromatography. The purification process was performed by step, DEAE-Sephacel, Ulttrogel AcA 34 and heptylamine-Sepharose column chromatography. The chromatographic fractions were confirmed by GTP binding assay and SDS-polyacrylamide gel electrophoresis. Molecular weight of Goa was revealed 39,000 dalton and GR 36,000 dalton. One more step of heptylamine-Sepharose was enforced to purify the GTP binding protein. Finally I gained the GTP binding protein isolated subtype of Goalpha and Gbeta.
