Comparison of an Automated Repetitive Sequence-based PCR Microbial Typing System with IS6110-Restriction Fragment Length Polymorphism for Epidemiologic Investigation of Clinical Mycobacterium tuberculosis Isolates in Korea.
10.3343/kjlm.2011.31.4.282
- Author:
Mi Hee JANG
1
;
Go Eun CHOI
;
Bo Moon SHIN
;
Seon Ho LEE
;
Sung Ryul KIM
;
Chulhun L CHANG
;
Jeong Man KIM
Author Information
1. Department of Laboratory Medicine, School of Medicine, Pusan National University, Yangsan, Korea. cchl@pusan.ac.kr
- Publication Type:Brief Communication ; Comparative Study ; Evaluation Studies
- Keywords:
Mycobacterium tuberculosis;
Repetitive sequence-based polymerase chain reaction;
DiversiLab Microbial Typing System
- MeSH:
Automation;
*Bacterial Typing Techniques;
*Epidemiologic Methods;
Genotype;
Humans;
Mycobacterium tuberculosis/*classification/genetics/isolation & purification;
*Polymerase Chain Reaction;
Polymorphism, Restriction Fragment Length;
Reagent Kits, Diagnostic;
Repetitive Sequences, Nucleic Acid;
Republic of Korea/epidemiology;
Tuberculosis/diagnosis/*epidemiology/microbiology
- From:The Korean Journal of Laboratory Medicine
2011;31(4):282-284
- CountryRepublic of Korea
- Language:English
-
Abstract:
Tuberculosis remains a severe public health problem worldwide. Presently, genotyping is used for conducting epidemiologic and clinical studies on tuberculosis cases. We evaluated the efficacy of the repetitive sequence-based PCR (rep-PCR)-based DiversiLab(TM) system (bioMerieux, France) over the IS6110-restriction fragment length polymorphism analysis for detecting Mycobacterium tuberculosis. In all, 89 clinical M. tuberculosis isolates collected nationwide from Korea were used. The DiversiLab system allocated the 89 isolates to 8 groups with 1 unique isolate when a similarity level of 95% was applied. Seventy-six isolates of the Beijing family and 13 isolates of non-Beijing family strains were irregularly distributed regardless of rep-PCR groups. The DiversiLab system generated a rapid, sensitive, and standardized result. It can be used to conduct molecular epidemiologic studies to identify clinical M. tuberculosis isolates in Korea.
