Proteomic Identification of Proteins Suggestive of Immune-Mediated Response or Neuronal Degeneration in Serum of Achalasia Patients.
- Author:
Seon Kyo IM
1
;
Mari YEO
;
Kwang Jae LEE
Author Information
1. Department of Gastroenterology, Ajou University School of Medicine, Suwon, Korea. kjleemd@hotmail.com
- Publication Type:Validation Studies ; Original Article
- Keywords:
Esophageal achalasia;
Immune response;
Neuronal degeneration;
Proteomics
- MeSH:
alpha-Macroglobulins;
Complement C3;
Complement System Proteins;
Cyclin-Dependent Kinase 5;
Enzyme-Linked Immunosorbent Assay;
Esophageal Achalasia;
Ganglion Cysts;
Humans;
Neurons;
Prealbumin;
Proteins;
Proteomics
- From:Gut and Liver
2013;7(4):411-416
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS: The primary pathophysiologic abnormality in achalasia is known to be a loss of inhibitory myenteric ganglion cells, which may result from an immune-mediated response or neuronal degeneration. The aim of this study was to identify proteins suggestive of an immune-mediated response or neuronal degeneration in the serum of achalasia patients using a proteomic analysis. METHODS: Blood samples were collected from five symptomatic achalasia patients and five sex- and age-matched healthy controls. Serum proteomic analysis was conducted, and the protein spots were identified using matrix-assisted laser desorption ionization/time-of-flight and a proteomics analyzer. The serum level of C3 was measured by enzyme-linked immunosorbent assay in nine patients with achalasia and 18 sex- and age-matched healthy controls. RESULTS: Of the 658 matched protein spots, 28 spots were up-regulated over 2-fold in the serum from achalasia patients compared with that from controls. The up-regulated proteins included complement C4B5, complement C3, cyclin-dependent kinase 5, transthyretin, and alpha 2 macroglobulin. The serum levels of C3 in achalasia patients were significantly higher than those of controls. CONCLUSIONS: The serum proteomic analysis of achalasia patients suggests an immune-mediated response or neuronal degeneration. Further validation studies in larger samples and the esophageal tissue of achalasia patients are required.
