Bacteroides nordii and Bacteroides salyersiae Isolated from Post-operative Peritonitis Patients.
- Author:
Jihoon G YOON
1
;
Hyunsoo KIM
;
Yangsoon LEE
;
Myungsook KIM
;
Dongeun YONG
;
Seok Hoon JEONG
;
Kyungwon LEE
Author Information
- Publication Type:Case Report
- Keywords: Bacteroides fragilis group; Bacteroides nordii; Bacteroides salyersiae; Spectrometry; Mass; Matrix-assisted laser desorption-ionization
- MeSH: Ascitic Fluid; Bacteroides fragilis; Bacteroides*; Humans; Mass Spectrometry; Peritonitis*; Spectrum Analysis
- From:Laboratory Medicine Online 2016;6(2):111-115
- CountryRepublic of Korea
- Language:Korean
- Abstract: In this study, we report three cases in which two species of the Bacteroides fragilis group, 'Bacteroides nordii' and 'Bacteroides salyersiae', were isolated from peritoneal fluid cultures from post-operative peritonitis patients. The two species of the B. fragilis group were initially misidentified as B. fragilis/Bacteroides stercoris and Bacteroides ovatus by Rapid ID 32A (bioMérieux, France), and finally confirmed as B. nordii and B. salyersiae using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16s rRNA sequencing. For the identification of anaerobes, particularly B. fragilis group organisms, MALDI-TOF MS is a useful method not only because of its concordance with 16S rRNA sequencing results, but also because of its rapidity and simple procedure.
