Diagnostic Utility of Multiprobe Fluorescence in situ Hybridization Assay for Detecting Cytogenetic Aberrations in Acute Leukemia.
10.3343/alm.2014.34.3.198
- Author:
Bo Ram KIM
1
;
Jae Lim CHOI
;
Ji Eun KIM
;
Kwang Sook WOO
;
Kyeong Hee KIM
;
Jeong Man KIM
;
Sung Hyun KIM
;
Jin Yeong HAN
Author Information
1. Department of Laboratory Medicine, Dong-A University College of Medicine, Busan, Korea. jyhan@dau.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Multiprobe FISH;
Acute leukemia;
Cytogenetic aberration
- MeSH:
Acute Disease;
Adolescent;
Adult;
Aged;
Child;
Child, Preschool;
*Chromosome Banding;
Core Binding Factor Alpha 2 Subunit/genetics;
Gene Deletion;
Humans;
*In Situ Hybridization, Fluorescence;
Karyotyping;
Leukemia/*diagnosis;
Leukemia, Myeloid, Acute/diagnosis;
Male;
Middle Aged;
Precursor Cell Lymphoblastic Leukemia-Lymphoma/diagnosis;
Proto-Oncogene Proteins c-ets/genetics;
Repressor Proteins/genetics;
Translocation, Genetic;
Tumor Suppressor Protein p53/genetics;
Young Adult
- From:Annals of Laboratory Medicine
2014;34(3):198-202
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Specific cytogenetic aberrations detected by conventional karyotyping or FISH play a major role in the diagnosis, prognosis, and treatment of patients with acute leukemia. The FISH technique enhances the capacity of conventional karyotyping to detect subtle chromosomal aberrations. Multiprobe FISH assay (Cytocell, UK) can hybridize multiple probes to a single slide, thereby increasing the detection rate of cytogenetic aberrations. This study aimed to evaluate multiprobe FISH in detecting cytogenetic abnormalities in acute leukemia. METHODS: Thirty newly diagnosed acute leukemia patients who attended the hematology clinic at Dong-A University Hospital from October 2008 to October 2012 were enrolled in the study. The multiprobe FISH results were compared with those of G-banding. RESULTS: Multiprobe FISH detected the chromosomal aberrations identified by G-banding, as well as additional aberrations in 6 of 30 (20.0%) cases, which included ETV6/RUNX1 translocation, p16 deletion, TP53 deletion, and IGH break-apart. CONCLUSIONS: The multiprobe FISH assay was a more sensitive and reliable technique compared with G-banding. It was also more cost-effective and yielded faster results.
