Toll-like receptor 2 expression on monocytes from patients with BCG vaccine-associated suppurative lymphadenitis.
10.3345/kjp.2009.52.6.667
- Author:
Hyun Joo OH
1
;
Kyung Sue SHIN
Author Information
1. Department of Pediatrics, Cheju National University School of Medicine, Jeju, Korea. kyungsue@jeju.ac.kr
- Publication Type:Original Article
- Keywords:
Mycobacterium bovis bacillus Calmette-Gurin;
BCG vaccine-associated suppurative lymphadenitis;
Toll-like receptor 2;
Polymorphism
- MeSH:
Cell Separation;
Enzyme-Linked Immunosorbent Assay;
Humans;
Interleukin-6;
Lymphadenitis;
Magnets;
Monocytes;
Mycobacterium bovis;
RNA, Messenger;
Toll-Like Receptor 2;
Toll-Like Receptors;
Tumor Necrosis Factor-alpha
- From:Korean Journal of Pediatrics
2009;52(6):667-673
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Toll-like receptor 2 (TLR2) is critical in the immune response to mycobacterial infections. The purpose of this study was to analyze TLR2 surface expressions and TLR2-mediated tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) production in patients with BCG vaccine-associated suppurative lymphadenitis. METHODS: Peripheral monocytes were separated from 16 patients with BCG vaccine-associated suppurative lymphadenitis and 10 healthy controls using a magnet cell isolation kit. Monocytes (1x10(6) cells/well) were incubated with a constant amount of Pam(3)CSK(4) (100 microgram/mL) for 24 hours. TLR2 surface expression on monocytes was analyzed by FACS analysis and TLR-2 mRNA expression was determined by RT-PCR. TLR2-mediated TNF-alpha and IL-6 production were measured by ELISA. RESULTS: In patients with BCG vaccine-associated suppurative lymphadenitis, low TLR2 expression on monocytes (3.39+/-1.2% versus 4.64+/-2.6%) together with significantly lower TLR2 mRNA expression than in the healthy controls was seen after Pam(3)CSK(4) stimulation. TLR2-mediated TNF-alpha and IL-6 production in patients with BCG vaccine-associated suppurative lymphadenitis (TNF-alpha, 775.5+/-60.8 pg/mL; IL-6, 4,645.8+/-583.9 pg/mL) were also lesser than that in healthy controls (TNF-alpha, 1,098.5+/-94.3 pg/mL; IL-6, 6,696.3+/-544.3 pg/mL). CONCLUSION: These findings suggest that low TLR2 expression on monocytes might be associated with increased susceptibility to BCG vaccine-associated suppurative lymphadenitis.
